Based on vibrational imaging (Stimulated Raman Coherent for CARS or Stimulated Raman Scattering microscopy), the new equipment acquired by the FR AIB imaging facility will make it possible to obtain images of chemical compounds within biological samples, without prior labelling. This is a point of particular importance when it comes to distinguishing lipids...
The RAMAN CARS/SRS technology...
The new microscope, installed and now fully operational, makes it possible to highlight chemical compounds within thick, unstained, living biological samples and thus to draw up high-resolution chemical mappings within the sample.
Particularly effective at revealing lipids and discriminating between compounds on the basis of their carbon chains, this new instrument will also be capable of localising rare chemical elements. Equipped with several detectors, it is ultimately able to couple CARS or SRS acquisitions with «traditional» fluorescence, multi-photon or second-harmonic acquisitions in order to superimpose the various data sources and thus characterise the sample in great detail.
...and the associated expertise
To facilitate the implementation of this new technology, an engineer will join the team in April 2022.
CARS = Coherent Anti-Stokes Raman Scattering (= diffusion Raman anti-Stokes cohérente)
SRS = Stimulated Raman Scattering (= stimulated Raman scattering)
