HIGH-THROUGHPUT MULTIPARAMETRIC IMAGING AND ANALYSIS

For high-throughput cell population observation and analysis

High-throughput imaging encompasses instruments dedicated to the acquisition, measurement and analysis of cellular content from various media (slides or plates up to 384 wells, etc.). They integrate an automated confocal or widefield fluorescence microscope for the analysis of fixed or living cells in a controlled environment. Intuitive, these resources offer the possibility of analysing multiple structural and functional cellular parameters in parallel. A dedicated server enables data storage, management and analysis.

Booking

Services

  • XYZT acquisition, widefield or confocal, multicolour fluorescence or transmitted light,
  • Live or fixed samples
  • Up to 384 samples in a single run,
  • Analysis of fluorescence parameters,
  • Analytical capabilities for a wide range of research and high-content screening (HCS) applications: cell biology, immunostaining, cytotoxicity, pharmacokinetics, oncology, etc.
  • Off-site management, data storage and analytics,

Access method

  • With assistance
  • Autonomy (after training)
  • As service delivery

HIGH CONTENT IMAGING resources

Designation

Features

Plateau

OPERETTA CLS

High Content Imaging system

PerkinElmer

5x, 20x, 40x objectives
Spinning disk confocal mode
Transmission mode
6.45 × 6.45 µm pixels
Thermo-regulated chamber (37°C and 3 to 8.5% CO2)

CRCT – Oncopole

Revvity

Air objectives 1.25x, 5, 10, 20x / water: 20x 40x 63x
Rotating disc
4.7 Megapixels from 6.5 × 6.5 µm
Automation: Serial and live imaging, capacity for up to 1536-well plates
4 lasers 405nm, 488nm, 560nm red, 647nm far red
4 simultaneous CMOS cameras
Thermo-regulated chamber (37-42°C & CO2 from 1 to 10%)

IRSD – Purpan University Hospital

Cell Discoverer 7

High Content Imaging System

ZEISS

Automated “medium-throughput”, high-content acquisitions (mosaic or multi-position, automated water immersion management, acquisition feedback control capability via image analysis – Zen Desk software).
Multi-supports (96-well plates, microscope slides, Petri dishes, etc.)
3 self-corrected objectives for multi-scale imaging: 3 objectives: 5X/NA 0.4 air WD 5 mm; Plan-Apo 20X/NA 0.7 air WD 1.4 mm; Plan-Apo 50X/NA 1.2 water WD 0.8 mm, with the added flexibility of 3 Optovars (0.5X, 1X and 2X).

Transmission mode (bright-field and PGC), widefield epifluorescence mode with 4 Colibri LEDs (385 nm, 475 nm, 567 nm red, 625 far-red) and quad-band (speed) or single-band (specificity) emission filters for imaging DAPI, eGFP, Cy3 and Cy5 type fluorochromes.
Axiocam 712 mono R2 camera (3.45 × 3.45 µm pixels)
Temperature control (30 to 45°C) and CO2 (0 to 8 %) for long-term live imaging

CBI / LITC – Campus UT

Operetta
High Content Imaging System PERKIN ELMER

x2, x10, x20 & x40 objectives
Transmission mode
6.45 × 6.45 µm pixels
Temperature-controlled chamber (37°C & CO2 from 3 to 8,5%)

RESTORE – Oncopole

Booking