Tomography

Understand the volume using a TEM.

Electron tomography makes it possible to recreate volumes at the nanoscale using a Transmission Electron Microscope (TEM). Much like a medical CT scan, images of the same object are taken from different angles. Imaging software is then used to reconstruct the sample's volume and visualise it in 3D. Sample preparation is identical to that for observation using a TEM.

Booking

Services

  • 3D reconstruction of objects up to 300nm thick

Access method

  • With assistance
  • As service delivery

Transmission electron microscopy resources

Designation

Plateau

Plateau

Thermo Fisher Talos Arctica

x60 to x1,100,000, equipped with cryo-tomography and high-resolution cryo-microscopy

METi – Campus UPS

Jeol JEM 2100

x 50 to x 1,000,000, equipped with cryo-electron microscopy, tomography & energy filter

METi – Campus UPS

Jeol JEM 1400

50x to 1,200,000x equipped with tomography

METi – Campus UPS

A few publications with these resources

  • The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography. Nat Commun., 10(1):497. Delavoie F., Soldan V., Rinaldi D., Dauxois J-Y., Gleizes P-E. (2019)
Booking