Research work carried out in several laboratories has led to the development of microscopes using light-sheet excitation, also known as SPIM (Selective Plane Illumination Microscopy). This technique allows for rapid, three-dimensional and deep optical sectioning for the observation of living matter.
Following the same principle, the MacroSPIM, based on light-sheet excitation and developed with the support of the IRB, enables the visualisation – following clearing – of tissues, organoids and entire organs via rapid optical sectioning, also in 3D and in depth. The fields of application are extremely numerous and concern both animal and plant subjects.
Designation
Features
Plateau
SPIM – light sheet microscope
Team of three continuous wave lasers (491, 532, 595nm)
Temperature-controlled chamber supplied with CO2. Small samples (e.g. cells, tissues, etc.)
Restore – UT3 Dental
Macro-SPIM – unavailable
Equipped with four continuous-wave lasers (405, 488, 561, 642 nm). Samples of several cm3 (e.g., organs…)
Restore – UT3 Dental
Zeiss Lightsheet 7
System of four continuous-wave lasers (405, 488, 561, 638 nm). Samples from µm to cm (spheroids, organoids, tissues and embryos). Temperature-controlled chamber.
Restore – Oncopôle
Mouse intestine – Restore
Mouse intestine – Restore
Plant – Restore
To find out more about the developments in light-sheet imaging carried out by the Restore teams, please visit the activity page Imaging R&D.
