CYTOMÈTRES

For the analysis and characterisation of cell populations

Flow cytometry is the quintessential high-throughput analysis technique. It enables rapid cell-by-cell analysis based on criteria of size, cellular content (granularity) and fluorescence. Depending on the equipment used, the analysis can be conducted based on criteria measuring up to 40 parameters simultaneously on cells in suspension. 

Booking

Services

  • Multi-membrane and intracellular labelling (17 colours)
  • Cellular functions (apoptosis, proliferation, cell cycle, calcium flux, ploidy, membrane potential, etc.)
  • Analysis and characterisation of stem cells
  • Multiplex molecular analysis & fluorescent cell barcoding
  • Analysis and quantification of molecular interactions (FRET)
  • Transfection efficiency analysis
  • Characterisation of animal cells, plant cells, bacteria, yeasts, nanoparticles, organelles...
  • Image acquisition of each analysed cell
  • dedicated analysis stations and software available to users

Access method

  • With assistance
  • Autonomy (after training)
  • As service delivery

Flow cytometry resources

Designation

Number & type of lasers

Concurrent parameters

Features

Plateau

AURORA

4 visible & 1 UV

>40

Spectral cytometer

CRCT – Oncopole

LSRT Fortessa X-20

4 visible & 1 UV

18

Plate handler

CRCT – Oncopole

LSRII

3 visible

11

–

CRCT – Oncopole

MACSQuant 10

3 visible

8

Plate and tube handler

CRCT – Oncopole

MACSQUANT VYB

3 visible

8

Plate and tube handler

CRCT – Oncopole

LSRT Fortessa X-20

4 visible & 1 UV

18

–

Infinity – CHU Purpan

Spectral ID7000

4 visible & 1 UV

>40

Spectral cytometer and plate-based automated analysis

Infinity – CHU Purpan

BD FACSymphony

4 visible & 1 UV

30

–

Infinity – CHU Purpan

Fortessa

4 visible

16

–

Infinity – CHU Purpan

ImageStreamX Mark II

3 visible

6

Imaging flow cytometer

Infinity – CHU Purpan

BD Fortessa

4 visible

13

–

I2MC – CHU Rangueil

Aurora CS

3 visible & 1 UV

>40

–

I2MC – CHU Rangueil

LSR Fortessa X20

4 visible & 1UV

18

HTS plate handler

IPBS – Rangueil

BD C6 Accuri

2 visible

4

All types of tubes

IPBS – Rangueil

Northern Lights (Cytek)

3 visible

30

Spectral cytometer and plate loader

IPBS – Rangueil

BD FACS AriaFusion (ASB3)

3 visible

14

Containment Level 3

IPBS – Rangueil

LSR Fortessa X-20

4 visible & 1UV

18

–

RESTORE – Oncopole

Miltenyi MACSQUANT Q10

2 visible & 1 UV

10

Plate and tube handler

RESTORE – Oncopole

ImageStreamX Mark II

3 visible

6

Imaging flow cytometer

RESTORE – Oncopole