Characterising and observing cell populations | |
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The ImageStreamX is an interdisciplinary instrument that bridges flow cytometry and microscopy. It combines the advantages of both technologies, thereby bringing the power of statistical flow cytometry data to a large number of cellular morphological parameters. This system generates images of cells in flow using magnifications ranging from 20x to 60x. It caters to a wide range of applications (spot counting, nuclear translocation, internalisation, etc.) across various fields: immunology, oncology, parasitology, microbiology, etc.
Technical specifications:
- a Brightfield laser enabling the measurement of cell size and morphology,
- a 785 nm darkfield laser providing information on the cell's content and granularity (side scatter),
- 3 lasers enabling the quantification and localisation of markers of interest in each detection channel: 405nm, 488nm and 642nm,
- Equipped with a CCD camera, this configuration makes it possible to collect simultaneously up to 6 images of each cell,
- 3 objective lens sizes: 20x, 40x and 60x,
- 2 associated software programmes: INSPIRE (acquisition) and IDEAS (statistical analysis).
Applications:
- morphological analysis,
- internalisation,
- cell signalling, nuclear translocation,
- colocalisation,
- cellular interactions,
- cell cycle,
- apoptosis,
- spot counting,
- autophagy and cell death,
- numerous emerging applications (FISH, FRET, etc.)
Access mode:
- With assistance
- Collaborative R&D project
- As service delivery
Our cytometers image resources
Designation | Plateau | |
| Image streamX | Infinity- Purpan | |
| Image streamX | Restore – Oncopôle |
