The Opera Phenix available on the IRSD Organoid platform is a spinning disk confocal microscope. It enables the rapid and fully automated acquisition of a wide variety of live or fixed samples (organoids, cells, tissues, bacteria, etc.) on plates or slides. Thanks to the presence of 4 cameras, it is possible to acquire 4 fluorescence channels simultaneously (DAPI, green, red and far red) as well as brightfield transmission.
The applications of this microscope are extremely varied (quantification, morphological studies, tracking, FISH, translocation, mitotic index calculation, etc.). The modules available for analysis make it possible to obtain highly robust data quickly and easily. In addition, the organoid platform provides a culture room for users who may need one.
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- A: Mouse colon crypts, Nuclei (DAPI), EPCAM (green), Ki67 (red), : A Edir, Eq 01, IRSD:
- B: Colon organoids, Nuclei (DAPI), Muc2 (green), Phalloidin (red), D Sagnat, Organoid Platform, IRSD
- C: Epididymis organoids, Nuclei (DAPI), CD49 (green), Androgen receptor (red) G Perez, E Huyghe (DEFE)
- D Comparative analysis of the effects of a drug on granule distribution and exocytosis in NK-92 cells. German et al., 2021, Cell Reports 36
Some publications produced using this instrument:
- LFA-1 nanoclusters integrate TCR stimulation strength to tune T-cell cytotoxic activity – Lacouture et al., Nat Commun. 2024
- Kinetic measurements of human CD8+ T cell cytotoxic activity in a 384-well plate format – Lacouture et al., Methods Cell Biol. 2023
- Metrics of 2D immunological synapses in human T cells via high-content confocal cell imaging – Prunier et al., Methods Cell Biol. 2023:
- Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukaemia – Rey-Barroso et al., Haematologica. 2023 Jun 29.
Article published as part of the highlights GENOTOUL
