The Infinity imaging platform (formerly CPTP) has acquired, thanks to support granted by the region as part of the «Octopus» project, a multi-photon confocal microscope enabling rapid acquisitions over a wide field of view: the BRUKER 2P+.
This motorised upright stand microscope enables multi-colour cellular and tissue observation, thanks to 4 non-descanned detectors (NDDs) allowing better light sensitivity. A Chameleon Discovery femtosecond pulsed laser, tunable between 680 and 1340 nm, makes it possible to excite a wide spectrum of fluorochromes and conduct observations in non-linear optics (SHG, THG). It is thus possible to detect cellular components such as collagen or lipids without prior labelling of the sample, resulting in a valuable time-saving during sample preparation.
This instrument, available to the entire scientific community, will make it possible to lift numerous technological barriers.
- Technical specifications: LASER Chameleon Discovery (680nm-1340nm), 20X immersion objective NA: 1, Galvo and resonant scanner, 400µm piezo Z, 4 GaAsp NDDs, equipped for tissue explant and small animal microscopy.
- Associated software: PrairieView v5.5
- Location: Cellular Imaging Platform, Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), Inserm UMR1291
- Persons in charge: Sophie Allart (05.62.74.45.78) / Lhorane Lobjois (05.31.54.79.01)
Instrument financed under the Octopus project by the Occitanie Region
