TRANSMISSION ELECTRON MICROSCOPE

Very high resolution to observe and analyse the composition of elementary structures

As the name suggests, electron microscopy is not based on photons but on electrons. Transmission electron microscopy (TEM) therefore makes it possible to achieve a very high resolution (magnification from x 50 to x 1,200,000). A high-energy electron beam is transmitted through a thin specimen and the image is formed on a phosphorescent screen coupled to a digital camera. TEM allows numerous applications associated with various preparation techniques, including detailed observations of the internal structures of cells, bacteria, viruses, etc.

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Services

Access method

  • With assistance
  • Autonomy (after training)
  • As service delivery

Transmission electron microscope resources

Designation

Plateau

Plateau

Thermo Fisher Talos Arctica

x60 to x1,100,000, equipped with cryo-tomography and high-resolution cryo-microscopy

METi – Campus UPS

Jeol JEM 2100

×50 to ×1,000,000, equipped with cryo-electron microscopy, tomography and elemental analysis

METi – Campus UPS

Jeol JEM 1400

50x to 1,200,000x equipped with tomography

METi – Campus UPS

MET HT 7700 Hitachi

 x 200 to x 600,000, remote control

CMEAB – Rangueil

Some publications produced with the assistance of the instruments

  • Carron C, Balor S, Delavoie F, Plisson-Chastang C, Faubladier M, Gleizes PE, O’Donohue MF; J. Cell Science October 2012, Post-mitotic dynamics of pre-nucleolar bodies is driven by pre-rRNA processing.
  • Rath P, Saurel O, Czaplicki G, Tropis M, Daffé M, Ghazi A, Demange P, Milon A.. Cord factor (trehalose 6,6′-dimycolate) forms fully stable and non-permeable lipid bilayers required for a functional outer membrane. Biochim Biophys Acta. Sep. 2013.
  • Atomic force and electron microscopic-based study of sarcolemmal surface of living cardiomyocytes unveils unexpected mitochondrial shift in heart failure. Dague E, Genet G, Lachaize V, Guilbeau-Frugier C, Fauconnier J, Mias C, Payré B, Chopinet L, Alsteens D, Kasas S, Severac C, Thireau J, Heymes C, Honton B, Lacampagne A, Pathak A, Sénard JM, Galés C. J Mol Cell Cardiol, 2014, 74, 162-72.
  • 5,6-Epoxy-cholesterols contribute to the anticancer pharmacology of tamoxifen in breast cancer cells. Segala G1, de Medina P, Iuliano L, Zerbinati C, Paillasse MR, Noguer E, Dalenc F, Payré B, Jordan VC, Record M, Silvente-Poirot S, Poirot M. Biochem Pharmacol, 2013, 86(1), 175-89.
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