Chromatin structure and dynamic
Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)
AFM, mesure de force multi-échelles et multimodale
Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.
Analyse de la signalisation immunitaire à l’échelle de la cellule (Cytométrie en images – Analyse de la synapse immunologique par STED 3D)
T-cell cytotoxic function relies on the cooperation between the highly specific T-cell receptor (TCR) and integrins of co-stimulatory molecule at the immunological synapse. Super resolution 3D STED (Leica SP8, 775 nm) is used to study the oorganisation of these molecules during the activation of immune response. Image cytometer (ImageSteam) allows the quantification of molecules recruited […]
Organoides : de la préparation à l’analyse
Organoides (poumon, endometriose, digestif, tissu adipeux, moelle, peau, vessie, trompe de Fallope): analyse intégrative (préparation échantillon, acquisition -feuille de lumiere, confocal, biphoton, high content screening, analyse multiparametrique, imagerie 3D, analyses, cytométrie en flux et en images)