CONFOCAL MICROSCOPE

 

Confocal

One or two-photon – many available variants

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Telephone : +33 5 61 55 75 93
Confocal laser scanning microscopes were the first to come along. These extremely versatile (and therefore indispensable) tools equip each light microscopy facility in the TRI network. They capture the fluorescent signal only from the focal plane by getting rid of the stray fluorescence from the upper and lower planes. The point-by-point laser illumination provides great flexibility in acquisition, including the ability to zoom in on the image until it reaches the limit of optical resolution (several dozen nanometers). However, this high-resolution ability implies a certain slowness, which can be overcome by confocal resonant scanning systems, more sensitive sensors or spinning disk-confocal systems.
Laser scanning microscopes can be seperated into two groups: one- and two-photon microscopes. The latter can penetrate deeper into matter and achieve a better resolution.
limbe coroneta

Violet leaf (limbe corneta) observed with a confocal microscope

Services :

  • XYZT observation (multipositions) and in time
  • Multi-labeled images
  • UV lasers for nuclear labels (i.e. DAPI, Hoechst)
  • Thermoregulation and CO2 control for sample maintenance
  • Live or fixed samples
  • Spectral analysis with nanometer precision
  • Ultra-sensitive detectors (i.e. GAsP, hybrids)
  • FRAP, fluorescence anisotropy measurements
  • Image analysis and 3D reconstruction (Volocity, Metamorph, Imaris, Amira)

Access mode:

  • With assistance
  • In autonomy (after training)
  • As collaborative project

 

 

One-photon confocal microscopeCliquez ici pour prendre contact afin de réserver une ressource

 

Name
Characteristic techniques
Location
LEICA SP2Upright microscopeCBD – Campus UPS
Zeiss 710 BigInverted (microscope), UV laser, multi-positions, Thermoregulated and CO2 controlled chamber, GAsPCBD – Campus UPS
Leica SP8 resonantInverted (microscope), UV laser, multi-positions, hybrid detectorCBD – Campus UPS
Leica SP8 resonantUpright microscope, multi-positions, hybrid detectorCBD – Campus UPS
LEICA SP8Inverted (microscope), UV laser, multi-positionsCPTP – CHU Purpan
Zeiss 510 invInverted (microscope)CPTP – CHU Purpan
Zeiss 710 invInverted (microscope), UV laserCPTP – CHU Purpan
ZEISS LSM780Thermoregulation & CO2 control, GAsPI2MC – CHU Rangueil
Zeiss LSM510Inverted (microscope), thermoregulation & CO2 control, multi-positionsI2MC – CHU Rangueil
LEICA SP2 AOBSUpright microscope, UV laserFR3450 – Campus INRA Auzeville
LEICA SP8Upright microscope, UV laser, multi-positions, hybrid detectorFR3450 – Campus INRA Auzeville
LEICA SP8-2017Upright microscope, UV laser, multi-positionsFR3450 – Campus INRA Auzeville
ZEISS LSM 510 InvInverted (microscope)IPBS – Rangueil
TIRF OLYMPUS FV1000Inverted (microscope), thermoregulation & CO2 control, UV laserIPBS – Rangueil

 

 

Two-photons confocal microscopeCliquez ici pour prendre contact afin de réserver une ressource

 

Name
Characteristic techniques
Location
LEICA SP5Upright microscope, multi-positionsCBD – Campus UPS
Zeiss 7MPUpright microscopeCPTP – CHU Purpan
FLIM 7MPUpright microscope, thermoregulated, FLIMIPBS – Rangueil
FLIM LSM710Inverted microscope, thermoregulated and CO2 controlled chamber, FLIMIPBS – Rangueil
Lavision BiotechUpright & inverted microscope, thermoregulated, Biosecurity level / BSL3 – MoreIPBS – Rangueil

Some publications made thanks to these resources:

  • Barlerin D, Bessière G, Domingues J, Schuette M, Feuillet C, Peixoto A. Biosafety Level 3 setup for multiphoton microscopy in vivo – 2017 Sci Rep. 2017 Apr 3;7(1):571. Download
  • I. El Mourdi, A. Canivet, N.T. Joncker, E. Bellard & S. Allart. High Accuracy 4D Cell Tracking Into Explanted Skin Using Two-Photon Excitation Microscopy – Microscopy research and technique. 2015
  • High Accuracy 4D Cell Tracking Into Explanted Skin Using Two-Photon Excitation Microscopy El Mourdi, A. Canivet, N.T. Joncker, E. Bellard, S. Allart. Microscopy Research and Technique, 2015
  • Labernadie A, Bouissou A, Delobelle P, Balor S, Voituriez R, Proag A, Fourquaux I, Thibault C, Vieu C, Poincloux R*, Charrière GM*, Maridonneau-Parini I*. Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes. Nat Commun. 2014
  • Vérollet C, Souriant S, Bonnaud E, Jolicoeur P, Raynaud-Messina B, Kinnaer C, Fourquaux I, Imle A, Benichou S, Fackler OT, Poincloux R, Maridonneau-Parini I. HIV-1 reprograms the migration of macrophages. Blood. 2014
  • Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O. Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection. PLoS Pathog. 2014

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