CONFOCAL SPINNING DISK MICROSCOPE

 

Microscope Spinning disk

The ideal compromise between resolution and phototoxicity

Cliquez ici pour prendre contact afin de réserver une ressource

Telephone : +33 5 61 55 75 93
Spinning disk microscopes combine the speed of a wide-field microscope with the resolution of a confocal. In order to do so, this technique uses lasers to illuminate the samples and ultra-sensitive cameras to detect the fluorescent signal. Like a conventional confocal microscope, it can be used to observe thick samples with accuracy. Unlike a laser scanning confocal, the spinning disk illuminates the entire viewing area simultaneously, which reduces the acquisition time (routinely around 150 ms) and therefore the phototoxicity.

 

drosophile au spinning disk

Drosophila imaged by spinning disk microscopyk

Services:

  • XYZT acquisition in fluorescence and bright-field
  • Multi-labeling
  • Live 3D reconstruction
  • Dual camera for simultaneous acquisition of 2 colors

Access mode:

  • With assistance
  • In autonomy (after training)
  • As collaborative project

 

Microscopes “spinning-diskCliquez ici pour prendre contact afin de réserver une ressource

Name
Characteristic techniques
Location
Perkin Elmer, CSU X1 Upright microscope, 2 cameras FRAIB – Campus INRA Auzeville
Roper, CSU X1 Inverted, thermoregulated and CO2 controlled chamber, multipositions, FRAP, photoactivation with 375 nm laser, two camera CPTP – CHU Purpan
Andor, CSU 22 Inverted, 2 cameras, thermoregulated and CO2 controlled chamber, multipositions IBCG – Campus UPS

 

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