Counting photons to quantify spatial proximity between molecules

Cliquez ici pour prendre contact afin de réserver une ressource



Telephone : +33 5 61 17 59 00
These multi-photon confocal microscopes are equipped with FLIM imaging modules to produce lifetime images. In biology, this type of module is mainly used to measure the spatial proximity of membrane receptors previously labeled with fluorochromes. More generally, FLIM imaging is a prerequisite for any comparison of fluorescence intensities in a confocal image and therefore the analysis of spatial proximity of molecules.


Beckel & Hyckel

Left: conventional fluorescent image in black and white to show the position in the sample – Right: lifetime image – Graph: lifetime by photon counting


  • XYZT acquisition, multi-color, spectral separation
  • Interactions between molecules (FLIM function)
  • Multi-labeled images: 2D to 5D + DIC
  • Evaluation of molecular traffic between compartments (FRAP)
  • Fluorescence anisotropy measurements


Access mode:

  • With assistance
  • In autonomy (after training)
  • As collaborative project
  • As service delivery



Available Confocal FLIMs


Caractéristiques techniques
Multi-photon confocal microscope FLIM 7MPMounted on an upright microscope, thermoregulatedIPBS – Rangueil
Multi-photon confocal microscope FLIM 7MPFLIM LSM710Mounted on an inverted microscope, thermoregulated and CO2 controlled chamberIPBS – Rangueil
Mono-photon confocal microscope LEICA SP8 SMDMounted on inverted microscopeFR3450 – Campus INRA Auzeville

Cliquez ici pour prendre contact afin de réserver une ressource

Comments are closed.