CONFOCAL MICROSCOPE

 

Confocal

One or two-photon – many available variants
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Telephone : +33 5 61 55 75 93
Confocal laser scanning microscopes were the first to come along. These extremely versatile (and therefore indispensable) tools equip each light microscopy facility in the TRI network. They capture the fluorescent signal only from the focal plane by getting rid of the stray fluorescence from the upper and lower planes. The point-by-point laser illumination provides great flexibility in acquisition, including the ability to zoom in on the image until it reaches the limit of optical resolution (several dozen nanometers). However, this high-resolution ability implies a certain slowness, which can be overcome by confocal resonant scanning systems, more sensitive sensors or spinning disk-confocal systems.
Laser scanning microscopes can be seperated into two groups: one- and two-photon microscopes. The latter can penetrate deeper into matter and achieve a better resolution.
limbe coroneta

Violet leaf (limbe corneta) observed with a confocal microscope

Services :

  • XYZT observation (multipositions) and in time
  • Multi-labeled images
  • UV lasers for nuclear labels (i.e. DAPI, Hoechst)
  • Thermoregulation and CO2 control for sample maintenance
  • Live or fixed samples
  • Spectral analysis with nanometer precision
  • Ultra-sensitive detectors (i.e. GAsP, hybrids)
  • FRAP, fluorescence anisotropy measurements
  • Image analysis and 3D reconstruction (Volocity, Metamorph, Imaris, Amira)

Access mode:

  • With assistance
  • In autonomy (after training)
  • As collaborative project

 

 

One-photon confocal microscopeCliquez ici pour prendre contact afin de réserver une ressource

 

 

Name
Characteristic techniques
Location
LEICA SP2 Upright microscope CBI – UT3 Paul Sabatier
Zeiss 710 Big Inverted (microscope), UV laser, multi-positions, Thermoregulated and CO2 controlled chamber, GAsP CBI – UT3 Paul Sabatier
Leica SP8 resonant Inverted (microscope), UV laser, multi-positions, hybrid detector CBI – UT3 Paul Sabatier
Leica SP8 resonant Upright microscope, multi-positions, hybrid detector CBI – UT3 Paul Sabatier
LEICA SP8 Inverted (microscope), UV laser, multi-positions Infinity – CHU Purpan
Zeiss 510 inv Inverted (microscope) Infinity – CHU Purpan
Zeiss 710 inv Inverted (microscope), UV laser Infinity – CHU Purpan
ZEISS LSM780 Thermoregulation & CO2 control, GAsP I2MC – CHU Rangueil
Zeiss LSM510 Inverted (microscope), thermoregulation & CO2 control, multi-positions I2MC – CHU Rangueil
LEICA SP2 AOBS Upright microscope, UV laser FR3450 – Campus INRA Auzeville
LEICA SP8 Upright microscope, UV laser, multi-positions, hybrid detector FR3450 – Campus INRA Auzeville
LEICA SP8-2017 Upright microscope, UV laser, multi-positions FR3450 – Campus INRA Auzeville
TIRF OLYMPUS FV1000 Inverted (microscope), thermoregulation & CO2 control, UV laser IPBS – Rangueil
Zeiss LSM 880 Upright, spectral analysis, multi-positions, mono or bi-photon, thermorégulation Restore – Oncopôle

 

Two-photons confocal microscopeCliquez ici pour prendre contact afin de réserver une ressource

 

Name
Characteristic techniques
Location
LEICA SP5 Upright microscope, multi-positions CBI – UT3 Paul Sabatier
Zeiss 7MP Upright microscope Infinity – CHU Purpan
FLIM 7MP Upright microscope, thermoregulated, FLIM IPBS – Rangueil
FLIM LSM710 Inverted microscope, thermoregulated and CO2 controlled chamber, FLIM IPBS – Rangueil
Lavision Biotech Upright & inverted microscope, thermoregulated, Biosecurity level / BSL3 – More IPBS – Rangueil
Bruker 2P+ Upright, resonant scanner, Z-piezo, multi-positions Infinity – CHU Purpan
Zeiss LSM 880 Upright, spectral analysis, multi-positions, mono or bi-photon, thermorégulation Restore – Oncopôle

Some publications made thanks to these resources:

  • Barlerin D, Bessière G, Domingues J, Schuette M, Feuillet C, Peixoto A. Biosafety Level 3 setup for multiphoton microscopy in vivo – 2017 Sci Rep. 2017 Apr 3;7(1):571. Download
  • I. El Mourdi, A. Canivet, N.T. Joncker, E. Bellard & S. Allart. High Accuracy 4D Cell Tracking Into Explanted Skin Using Two-Photon Excitation Microscopy – Microscopy research and technique. 2015
  • High Accuracy 4D Cell Tracking Into Explanted Skin Using Two-Photon Excitation Microscopy El Mourdi, A. Canivet, N.T. Joncker, E. Bellard, S. Allart. Microscopy Research and Technique, 2015
  • Labernadie A, Bouissou A, Delobelle P, Balor S, Voituriez R, Proag A, Fourquaux I, Thibault C, Vieu C, Poincloux R*, Charrière GM*, Maridonneau-Parini I*. Protrusion force microscopy reveals oscillatory force generation and mechanosensing activity of human macrophage podosomes. Nat Commun. 2014
  • Vérollet C, Souriant S, Bonnaud E, Jolicoeur P, Raynaud-Messina B, Kinnaer C, Fourquaux I, Imle A, Benichou S, Fackler OT, Poincloux R, Maridonneau-Parini I. HIV-1 reprograms the migration of macrophages. Blood. 2014
  • Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, Guerquin-Kern JL, Schnappinger D, Sório de Carvalho LP, Poquet Y, Neyrolles O. Mycobacterium tuberculosis Exploits Asparagine to Assimilate Nitrogen and Resist Acid Stress during Infection. PLoS Pathog. 2014

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