Sample preparation techniques for electron microscopy
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This page brings together all of the resources that use sample preparation techniques at low temperature for a better morphological preservation. The samples thus prepared can be observed by transmission (TEM) or scanning (SEM) cryomicroscopy.
- Ultra-rapid freezing by immersion in liquid ethane, or by high pressure freezing of whole samples (bacteria, viruses, particles, liposomes, etc.) in vitreous ice up to 200 µm in thickness
- Cryo-sections (CEMOVIS and Tokuyasu techniques)
- Automated cryo-substitution and resin embedding at very low temperatures
- Cryo-fracture and sublimation
- Transmission and scanning cryomicroscopy
- With assistance
- As service delivery
Available resources in sample preparation for electron microscopy
|Removable cryo chamber, cryosphere (to avoid contamination by frost), antistatic system & micromanipulator||METi – UPS Campus|
|Freeze substitution system|
|Fully automated processes, associated with an automated device to manage reagents||METi – UPS Campus|
|Cryofixateur high pressure cryo-fixation system|
|Freezing at -8000 to -12,000°/s under 2000 bars of pressure||METi – UPS Campus|
|Automated device for ultra-rapid freezing in ethane, equipped with a stereomicroscope||METi – UPS Campus|
|Cryo transfer chamber for MEB FEG Quanta 250 FEI|
for metallization, cryo-fracture and sublimation
|CMEAB, Rangueil faculty of medecine|
Some publications made thanks to these resources:
- Carron C, Balor S, Delavoie F, Plisson-Chastang C, Faubladier M, Gleizes PE, O’Donohue MF; J. Cell Science Octobre 2012, Post-mitotic dynamics of pre-nucleolar bodies is driven by pre-rRNA processing.
- Rath P, Saurel O, Czaplicki G, Tropis M, Daffé M, Ghazi A, Demange P, Milon A.. Cord factor (trehalose 6,6′-dimycolate) forms fully stable and non-permeable lipid bilayers required for a functional outer membrane. Biochim Biophys Acta. Sep. 2013.
- Artificial feeding of Varroa destructor through a chitosan membrane: a tool for studying the host-microparasite relationship. Tabart J1, Colin ME, Carayon JL, Tene N, Payre B, Vetillard A. Exp Appl Acarol, 2013,61(1), 107-18.