LIGHT SHEET MICROSCOPES (SPIM)

SPIM

Observing living specimen in 3D and in depth

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Telephone : +35 34 32 38 37
The work of R&D performed at Restore led to develop microscopes based on light sheet excitation or SPIM (Selective Plane Illumination Microscope). This technique not only enables fast optical sectioning of living specimen in 3D and in depth but also offers the possibility of multiview acquisitions, opening new perspectives in biological observation. On the same principle, the MacroSPIM, which is also based on light sheet excitation and built thanks to IRB partnership, makes it possible to view tissues and whole organs (after they have been made transparent) by rapid optical sectioning, again in 3D and in depth. The fields of application are numerous and can be used on both animals and plants.

Services :

  • A wide variety of fluorescence observation in 3D with a quasi-isotropic spatial resolution and high temporal resolution.
  • Living biological samples (from µm to several mm) or transparent fixed samples (from mm to several cm).
  • Applications in oncology, physiology, cell biology, developmental biology, plant biology and tissue engineering.

Observation, in depth, of mitosis by SPIM microscopy (in red: cells in mitosis; in blue: cells in interphase)

Additional information

 

Modes of access:

  • With assistance
  • As collaborative project

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LIGHT SHEET MICROSCOPES (SPIM)Equipped with three continuous lasers (491, 532, 595 nm)
Thermoregulated and CO2 controlled chamber. Small samples (e.g. cells, tissues …)		Restore – Paul Sabatier University UT3
MACRO-SPIMEquipped with four continuous lasers (405, 488, 561, 642 nm). Samples of several cm3 in size (e.g. organs …)Restore – Paul Sabatier University UT3
Light Sheet Z7 (Zeiss)Equipped with four continuous lasers (405, 488, 561, 638nm). Samples from several μm to cm. Thermoregulated controlled chamber.Restore – Paul Sabatier University UT3

Some movies made thanks to these tools

With SPIM

A journey into a spheroid – Restore

Cell in mitosis – Restore

With MacroSPIM

Mouse gut – Restore

Mouse gut – Restore

Plant – Restore

More informations

To learn more on last SPIM devloppements made by Restore teams, please visit R&D imaging webpage.

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