TIRF MICROSCOPY

 

microscopie TIRF

For the observation of surfaces

Cliquez ici pour prendre contact afin de réserver une ressource

 

 

 

Telephone : +33 5 61 55 75 93
TIRF (Total Internal Reflection Fluorescence) microscopy can image an optical section with a thickness in the range of 20 – 200 nm, as compared to 800 nm with a confocal. With TIRF, only the sample surface at the immediate proximity of the slide is observed. The instrument’s high axial resolution makes it a device of choice for membrane observations or cytoskeleton studies. This type of microscopy is performed on living samples, or on fixed samples in “PBS”, but it will not work on samples fixed and mounted between a slide and cover slip.

 

Stirf prise de vueervices :

  • Membrane receptor study
  • Exocytosis/endocytosis study
  • Cell adhesion/substrate study
  • Sub-membrane cytoskeletal study

 

TIRF MicroscopesCliquez ici pour prendre contact afin de réserver une ressource

 

Name

Characteristic techniques

Location

Olympus IX81 Inversé Inverted, thermoregulated and CO2 controlled chamber, multipositions &  Spinning Disk Infinity – CHU Purpan
Confocal TIRF Olympus FV1000 Inverted, thermoregulated and CO2 controlled chamber, FRAP & confocal IPBS – Rangueil

 

Comments are closed.