For the observation of surfaces |
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+33 5 61 55 75 93 |
TIRF (Total Internal Reflection Fluorescence) microscopy can image an optical section with a thickness in the range of 20 – 200 nm, as compared to 800 nm with a confocal. With TIRF, only the sample surface at the immediate proximity of the slide is observed. The instrument’s high axial resolution makes it a device of choice for membrane observations or cytoskeleton studies. This type of microscopy is performed on living samples, or on fixed samples in “PBS”, but it will not work on samples fixed and mounted between a slide and cover slip.
Services :
- Membrane receptor study
- Exocytosis/endocytosis study
- Cell adhesion/substrate study
- Sub-membrane cytoskeletal study
TIRF Microscopes
Name |
Characteristic techniques |
Location |
Olympus IX81 Inversé | Inverted, thermoregulated and CO2 controlled chamber, multipositions & Spinning Disk | Infinity – CHU Purpan |
Confocal TIRF Olympus FV1000 | Inverted, thermoregulated and CO2 controlled chamber, FRAP & confocal | IPBS – Rangueil |