SUPER-RESOLUTION NANOSCOPES OR MICROSCOPES

	For observations up to the molecular level
	+33 5 61 55 75 93

The diffraction limit of light, or Abbe’s limit, whose bottom value is around 0.2 μm, has always been thought as an absolute limit for photonic observation by photons. Indeed, below this limit, objects are nothing more than spots because the light reflected by each point cannot be distinguished from that of its neighbor. While they do use a beam of light like confocal microscopes, “nanoscopes” or “super-resolution microscopes” illuminate fluorochromes in a random (stochastic) or selective manner. Only the centers of the spots are then considered as being the exact location of the object. Finally, a higher resolution image is reconstructed by stacking the images.

 

Services:

• XYZT observation (multipositions) and in time using super-resolution techniques,
• Multi-labeled images,
• UV lasers for nuclear labels (i.e. DAPI, Hoechst),
• Live or fixed samples
• Spectral analysis with nanometer precision
• FRAP, fluorescence anisotropy measurements
• Image analysis and 3D reconstruction (Volocity, Metamorph, Imaris, Amira)

Modes of access:

• With assistance
• In autonomy (after training)
• As collaborative project

 

 

Super-resolution nanoscopes or microscopes

 

Name	Characteristic techniques	Location
STED 3X 775nm sur SP8	UV Laser, multi-positions	Infinity – CHU Purpan
dSTORM	Laser 640nm, TIRF	Infinity – CHU Purpan
NIKON N-STORM (SPT-PALM)	Lasers 405, 488, 561, 647nm, TIRF, SPT-PALM, STORM	LITC – Campus UPS
SYSTEME R&D	TIRF, SIM rapide multi-couleur	LITC – Campus UPS
Zeiss – ELYRA PS1	SIM, STORM, PALM	I2MC – Rangueil

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