CYTOMETERS

 

cytometre

For the analysis and characterization of cell populations

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Telephone : +33 5 31 54 79 11
Flow cytometry is the ultimate high throughput analysis technique. It allows rapid cell-by-cell analysis based on the criteria of size, cellular contents (granularity) and fluorescence. Depending on the equipment used, the analysis can be conducted by measuring up to 17 simultaneous parameters on cells in suspension. In addition, multiplexing techniques save time while standardizing and making the dosage and/or analysis uniform.

 

Services:

  • Thymic development

    Thymic development

    cytométrie

    Membrane and intracellular multi-labeling (17 colors)

  • Cell functions (apoptosis, proliferation, cell cycle, calcium flux, ploidy, membrane potential, etc.)
  • Analysis and characterization of stem cells
  • Molecular analysis in multiplex & fluorescent cell bar coding
  • Analysis and quantification of molecular interactions (FRET)
  • Analysis of transfection rate
  • Characterization of animal and plant cells, bacteria, yeast, nanoparticles, organelles, etc.
  • Image acquisition of each analyzed cell
  • PC and Mac provided for analysis

Access mode:

  • With assistance
  • In autonomy (after training)
  • As service delivery

CytometersCliquez ici pour prendre contact afin de réserver une ressource

Cytometer designation

Number and type of lasers
Simultaneous parameters
Other characteristics

Location

LSRII3 visibles & 1 UV13CPTP – CHU Purpan
Facscalibur2 visibles4CPTP – CHU Purpan
FC5002 visibles5Tube-based automated analysisCPTP – CHU Purpan
Fortessa4 visibles16Plate-based automated analysisCPTP – CHU Purpan
MACSQuantQ103 visibles8Plate & tube-based automated analysisCPTP – CHU Purpan
MACSQuantVYB3 visibles8Plate & tube-based automated analysisCPTP – CHU Purpan
ImageStreamX MarkII3 visibles6Cytometer with imagesCPTP – CHU Purpan
FacsCantoI2 visibles6Plate-based automated analysisCPTP – CHU Purpan
BD Fortessa4 visibles13I2MC – CHU Rangueil
BD FACSVerse1 visible4All kind of tubesI2MC – CHU Rangueil
BD FACScan1 visible3IPBS – Rangueil
BD LSRII3 visibles & 1 UV17IPBS – Rangueil
Facscalibur2 visibles4IPBS – Rangueil

*evt/s: events per second = (cells + impurities) sorted per second

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