CYTOMETERS

 

cytometre

For the analysis and characterization of cell populations

Cliquez ici pour prendre contact afin de réserver une ressource

 

 

Telephone : +33 5 31 54 79 11
Flow cytometry is the ultimate high throughput analysis technique. It allows rapid cell-by-cell analysis based on the criteria of size, cellular contents (granularity) and fluorescence. Depending on the equipment used, the analysis can be conducted by measuring up to 17 simultaneous parameters on cells in suspension. In addition, multiplexing techniques save time while standardizing and making the dosage and/or analysis uniform.

 

Services:

  • Thymic development

    Thymic development

    cytométrie

    Membrane and intracellular multi-labeling (17 colors)

  • Cell functions (apoptosis, proliferation, cell cycle, calcium flux, ploidy, membrane potential, etc.)
  • Analysis and characterization of stem cells
  • Molecular analysis in multiplex & fluorescent cell bar coding
  • Analysis and quantification of molecular interactions (FRET)
  • Analysis of transfection rate
  • Characterization of animal and plant cells, bacteria, yeast, nanoparticles, organelles, etc.
  • Image acquisition of each analyzed cell
  • PC and Mac provided for analysis

Access mode:

  • With assistance
  • In autonomy (after training)
  • As service delivery

CytometersCliquez ici pour prendre contact afin de réserver une ressource

Cytometer designation

Number and type of lasers
Simultaneous parameters
Other characteristics

Location

LSRII 3 visibles & 1 UV 13 CPTP – CHU Purpan
Facscalibur 2 visibles 4 CPTP – CHU Purpan
FC500 2 visibles 5 Tube-based automated analysis CPTP – CHU Purpan
Fortessa 4 visibles 16 Plate-based automated analysis CPTP – CHU Purpan
MACSQuantQ10 3 visibles 8 Plate & tube-based automated analysis CPTP – CHU Purpan
MACSQuantVYB 3 visibles 8 Plate & tube-based automated analysis CPTP – CHU Purpan
ImageStreamX MarkII 3 visibles 6 Cytometer with images CPTP – CHU Purpan
FacsCantoI 2 visibles 6 Plate-based automated analysis CPTP – CHU Purpan
BD Fortessa 4 visibles 13 I2MC – CHU Rangueil
BD FACSVerse 1 visible 4 All kind of tubes I2MC – CHU Rangueil
BD FACScan 1 visible 3 IPBS – Rangueil
BD LSRII 3 visibles & 1 UV 17 IPBS – Rangueil
Facscalibur 2 visibles 4 IPBS – Rangueil

*evt/s: events per second = (cells + impurities) sorted per second

Comments are closed