SUPER-RESOLUTION NANOSCOPES OR MICROSCOPES

Statif nSTORM -© D.Villa

For observations up to the molecular level

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Telephone : +33 5 61 55 75 93
The diffraction limit of light, or Abbe’s limit, whose bottom value is around 0.2 μm, has always been thought as an absolute limit for photonic observation by photons. Indeed, below this limit, objects are nothing more than spots because the light reflected by each point cannot be distinguished from that of its neighbor. While they do use a beam of light like confocal microscopes, “nanoscopes” or “super-resolution microscopes” illuminate fluorochromes in a random (stochastic) or selective manner. Only the centers of the spots are then considered as being the exact location of the object. Finally, a higher resolution image is reconstructed by stacking the images.

 

Filaments of actin with and without the super-resolution module. © CBI – T. Mangeat & A. Negash

Filaments of actin with and without the super-resolution module. © CBI – T. Mangeat & A. Negash

Services:

  • XYZT observation (multipositions) and in time using super-resolution techniques,
  • Multi-labeled images,
  • UV lasers for nuclear labels (i.e. DAPI, Hoechst),
  • Live or fixed samples
  • Spectral analysis with nanometer precision
  • FRAP, fluorescence anisotropy measurements
  • Image analysis and 3D reconstruction (Volocity, Metamorph, Imaris, Amira)

Modes of access:

  • With assistance
  • In autonomy (after training)
  • As collaborative project

 

 

Super-resolution nanoscopes or microscopesCliquez ici pour prendre contact afin de réserver une ressource

 

Name
Characteristic techniques
Location
STED 3X 775nm sur SP8 UV Laser, multi-positions CPTP – CHU Purpan
dSTORM Laser 640nm, TIRF CPTP – CHU Purpan
NIKON N-STORM (SPT-PALM) Lasers 405, 488, 561, 647nm, TIRF, SPT-PALM, STORM LITC – Campus UPS
SYSTEME R&D TIRF, SIM rapide multi-couleur LITC – Campus UPS
Zeiss – ELYRA PS1 SIM, STORM, PALM I2MC – Rangueil

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