Analyse structurale 3D à l’échelle atomique par cryo-microscopie électronique sur cellule et tissu (cryolamelle par cryo FIBSEM, CryoTEM, CryoSEM)
We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies […]
Microscopie en ASB3 (poumon infectés par des pathogenes de niveau 3)
Imagerie intravitale: Organes non infectés ou transparisés et tumeurs cancéreuses (en NSB1)
Bruker 2P+, Discovery laser (680 to 1340 nm), mouse anesthesy and stabulation. Cleared mouse organs (gut, lymph nodes..) and 2P microscopy.
Caractérisation de la réponse immunitaire et inflammatoire de la cellule au tissu par cytométrie en flux (panels optimisés clé en main – standardisation et analyse des données – cell barcoding) et imagerie confocale multiplex
To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).
Biologie appliquée à la cancérologie : préparation échantillons, analyse spatiale par imagerie multiplexée, cytométrie en flux sur cellules uniques
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various […]
Analyse multi-modales et multi-échelles des maladies inflammatoires, cardiovasculaires et dyslipidémiques
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various […]