{"id":10907,"date":"2025-08-25T16:47:09","date_gmt":"2025-08-25T14:47:09","guid":{"rendered":"https:\/\/trigenotoul.com\/?post_type=ressource&p=10907"},"modified":"2025-09-23T11:47:36","modified_gmt":"2025-09-23T09:47:36","slug":"super-resolution-nanoscopes-or-microscopes","status":"publish","type":"ressource","link":"https:\/\/trigenotoul.com\/en\/ressource\/super-resolution-nanoscopes-or-microscopes\/","title":{"rendered":"SUPER-RESOLUTION NANOSCOPES OR MICROSCOPES"},"content":{"rendered":"\t\t
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NANOSCOPES OU MICROSCOPES SUPER-RESOLUS<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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Pour des observations jusqu\u2019\u00e0 l\u2019\u00e9chelle de la mol\u00e9cule<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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La limite de diffraction de la lumi\u00e8re ou limite d\u2019Abbe, plancher fix\u00e9 aux alentours de 0.2\u00b5m, s\u2019est toujours pos\u00e9 comme une limite infranchissable pour l\u2019observation par les photons. En effet, en de\u00e7\u00e0 de cette limite, les objets ne sont plus que des taches car la lumi\u00e8re renvoy\u00e9e par chaque point ne peut \u00eatre distingu\u00e9e de son voisin. Bien qu\u2019utilisant \u00e0 l\u2019instar des microscopes confocaux un faisceau de lumi\u00e8re, les \u00ab nanoscopes \u00bb ou \u00ab microscopes \u00e0 super-r\u00e9solution \u00bb illuminent de mani\u00e8re al\u00e9atoire (stochastique) ou s\u00e9lective des fluorochromes. Seuls les centres des taches sont alors consid\u00e9r\u00e9s comme \u00e9tant l\u2019emplacement exact de l\u2019objet. Finalement l\u2019empilement des images permet de reconstruire une image de meilleure r\u00e9solution.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tR\u00e9servation<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Services<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tObservation XYZT (multipositions) et dans le temps avec techniques de super-r\u00e9solution,<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tImages multi-marquages<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tLasers UV pour marquage nucl\u00e9aires (type DAPI, Hoechst)<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\u00c9chantillons vivants ou fix\u00e9s<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tAnalyse spectrale au nanom\u00e8tre pr\u00e8s<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tFRAP, mesures d\u2019anisotropie de fluorescence<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
  • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tAnalyse d\u2019image et reconstruction 3D (volocity, metamorph, Imaris, Amira)<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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    Mode d'acc\u00e8s<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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    • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tAssistance<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
    • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tAutonomie (apr\u00e8s formation)<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t
    • \n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/path><\/svg>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\tPrestation de service<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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      Microscopes super-r\u00e9solus<\/h2>\t\t\t\t<\/div>\n\t\t\t\t
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      D\u00e9signation<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Caract\u00e9ristiques<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Plateau<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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      STED 3X 775nm sur SP8<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      laser UV, multi-positions<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Infinity \u2013 CHU Purpan<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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      dSTORM<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Laser 640nm, TIRF<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Infinity \u2013 CHU Purpan<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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      NIKON N-STORM (SPT-PALM)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      Lasers 405, 488, 561, 647nm, TIRF, SPT-PALM, STORM<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      LITC \u2013 Campus UPS<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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      SYSTEME R&D<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      TIRF, SIM rapide multi-couleur<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      LITC \u2013 Campus UPS<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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      Zeiss \u2013 ELYRA PS1<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      SIM, STORM, PALM<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t

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      I2MC \u2013 Rangueil<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

      Techniques that break with the resolution limit of light microscopy<\/p>","protected":false},"featured_media":0,"parent":0,"template":"","meta":{"_acf_changed":false},"domaine":[],"thematique":[],"class_list":["post-10907","ressource","type-ressource","status-publish","hentry"],"acf":[],"yoast_head":"\nSUPER-RESOLUTION NANOSCOPES OR MICROSCOPES - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/resource\/super-resolution-nanoscopes-or-microscopes\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SUPER-RESOLUTION NANOSCOPES OR MICROSCOPES - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Techniques that break with the resolution limit of light microscopy\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/resource\/super-resolution-nanoscopes-or-microscopes\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-23T09:47:36+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/ressource\\\/super-resolution-nanoscopes-or-microscopes\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/ressource\\\/super-resolution-nanoscopes-or-microscopes\\\/\",\"name\":\"SUPER-RESOLUTION NANOSCOPES OR MICROSCOPES - 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