{"id":7861,"date":"2024-04-15T11:01:34","date_gmt":"2024-04-15T09:01:34","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=7861"},"modified":"2024-04-15T11:01:34","modified_gmt":"2024-04-15T09:01:34","slug":"high-throughput-confocal-phenyx-lopera","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/lopera-phenyx-confocal-haut-debit\/","title":{"rendered":"THE PHENYX OPERA, the IRSD high-throughput confocal microscope"},"content":{"rendered":"<h5>The Opera Phenix available on the IRSD Organoid platform is a spinning disk confocal microscope. It enables the rapid and fully automated acquisition of a wide variety of live or fixed samples (organoids, cells, tissues, bacteria, etc.) on plates or slides. Thanks to the presence of 4 cameras, it is possible to acquire 4 fluorescence channels simultaneously (DAPI, green, red and far red) as well as brightfield transmission.<\/h5>\n<h5>The applications of this microscope are extremely varied (quantification, morphological studies, tracking, FISH, translocation, mitotic index calculation, etc.). The modules available for analysis make it possible to obtain highly robust data quickly and easily. In addition, the organoid platform provides a culture room for users who may need one.<\/h5>\n<p>&nbsp;<\/p>\n<hr \/>\n<blockquote>\n<h4>Contact &amp; booking:<\/h4>\n<p><a href=\"mailto:astrid.canivet@inserm.fr,david.sagnat@inserm.fr?subject=TRI-Genotoul\"><strong>Astrid Canivet &amp; David Sagnat<\/strong><\/a><\/p><\/blockquote>\n<hr \/>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter size-full wp-image-7862\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1.jpg\" alt=\"Opera Phenix organoids\" width=\"2432\" height=\"1366\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1.jpg 2432w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-300x169.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-1024x575.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-768x431.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-1536x863.jpg 1536w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-2048x1150.jpg 2048w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-623x350.jpg 623w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-150x84.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/04\/operaphenyx-1-1320x741.jpg 1320w\" sizes=\"(max-width: 2432px) 100vw, 2432px\" \/><\/p>\n<ul>\n<li><em>A: Mouse colon crypts, Nuclei (DAPI), EPCAM (green), Ki67 (red), : A Edir, Eq 01, IRSD:<\/em><\/li>\n<li><em>B: Colon organoids, Nuclei (DAPI), Muc2 (green), Phalloidin (red), D Sagnat, Organoid Platform, IRSD<\/em><\/li>\n<li><em>C: Epididymis organoids, Nuclei (DAPI), CD49 (green), Androgen receptor (red) G Perez, E Huyghe (DEFE)<\/em><\/li>\n<li><em>D Comparative analysis of the effects of a drug on granule distribution and exocytosis in NK-92 cells. German et al., 2021, Cell Reports 36<\/em><\/li>\n<\/ul>\n<hr \/>\n<blockquote><p><strong>Some publications produced using this instrument:<\/strong><\/p>\n<ul>\n<li>LFA-1 nanoclusters integrate TCR stimulation strength to tune T-cell cytotoxic activity \u2013 Lacouture et al., Nat Commun. 2024<\/li>\n<li>Kinetic measurements of human CD8+ T cell cytotoxic activity in a 384-well plate format \u2013 Lacouture et al., Methods Cell Biol. 2023<\/li>\n<li>Metrics of 2D immunological synapses in human T cells via high-content confocal cell imaging \u2013 Prunier et al., Methods Cell Biol. 2023:<\/li>\n<li>Lymphocyte migration and retention properties affected by ibrutinib in chronic lymphocytic leukaemia \u2013 Rey-Barroso  et al., Haematologica. 2023 Jun 29.<\/li>\n<\/ul>\n<\/blockquote>\n<hr \/>\n<p><em>Article published as part of the highlights <a href=\"https:\/\/www.genotoul.fr\/\" target=\"_blank\" rel=\"noopener\">GENOTOUL<\/a><\/em><\/p>","protected":false},"excerpt":{"rendered":"<p>L\u2019Opera Phenix pr\u00e9sent sur la plateforme Organo\u00efdes de l\u2019IRSD est un microscope confocal de type spinning disk. Il permet l\u2019acquisition d\u2019une grande vari\u00e9t\u00e9 d\u2019\u00e9chantillons vivant ou fix\u00e9s (organo\u00efdes, cellules, tissus, bact\u00e9rie\u2026) sur plaques ou lames de mani\u00e8re compl\u00e8tement automatis\u00e9e et rapide. Gr\u00e2ce \u00e0 la pr\u00e9sence de 4 cam\u00e9ras, il est possible d\u2019acqu\u00e9rir 4 canaux de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7862,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7861","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>L&#039;OPERA PHENYX, le microscope confocal \u00e0 haut d\u00e9bit de l&#039;IRSD - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/high-throughput-confocal-phenyx-lopera\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"L&#039;OPERA PHENYX, le microscope confocal \u00e0 haut d\u00e9bit de l&#039;IRSD - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"L\u2019Opera Phenix pr\u00e9sent sur la plateforme Organo\u00efdes de l\u2019IRSD est un microscope confocal de type spinning disk. Il permet l\u2019acquisition d\u2019une grande vari\u00e9t\u00e9 d\u2019\u00e9chantillons vivant ou fix\u00e9s (organo\u00efdes, cellules, tissus, bact\u00e9rie\u2026) sur plaques ou lames de mani\u00e8re compl\u00e8tement automatis\u00e9e et rapide. 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