{"id":7820,"date":"2024-03-18T13:52:51","date_gmt":"2024-03-18T12:52:51","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=7820"},"modified":"2025-11-20T15:54:57","modified_gmt":"2025-11-20T14:54:57","slug":"training-in-photonic-and-electronic-microscopy-cytometry-data-analysis","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/","title":{"rendered":"Training: Light and electron microscopy, cytometry and data analysis"},"content":{"rendered":"<h5><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-7822 alignleft\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2024\/03\/CNRS_RS-1.jpg\" alt=\"CNRS Formation Entreprises\" width=\"308\" height=\"163\" \/><\/h5>\n<h5>Combining alternating theoretical lectures (50 %) and practical sessions (50 %), this intensive training course is designed for company personnel wishing to improve their knowledge and skills regarding the acquisition and interpretation of images on various types of microscopes and cytometers. Taking place over 4 and a half days, this intensive event will combine lectures (limited to 10 trainees), practical sessions (in sub-groups of a maximum of 4 people with one or two instructors) and will be interspersed with exercises designed to support trainees in their progress.<\/h5>\n<p>&nbsp;<\/p>\n<h3 style=\"text-align: justify;\">OBJECTIVES<\/h3>\n<ul style=\"text-align: justify;\">\n<li>Gain theoretical foundations in light and electron microscopy, cytometry and data analysis,<\/li>\n<li>Know the possible applications for each technology,<\/li>\n<li>Understanding the practical aspect of these methods<\/li>\n<\/ul>\n<hr \/>\n<h3 style=\"text-align: justify;\">PUBLIC &amp; MODALITIES<\/h3>\n<ul>\n<li style=\"text-align: justify;\"><strong>Public<\/strong> Researchers, engineers and technicians wishing to discover all of these technologies<\/li>\n<li><strong>Prerequisite:<\/strong> None, except for a clearly stated interest in in vivo optical imaging<\/li>\n<li style=\"text-align: justify;\"><strong>Staff numbers:<\/strong> Maximum of 10 trainees, practical sessions in sub-groups of maximum 4 trainees with 1 instructor per sub-group<\/li>\n<li style=\"text-align: justify;\"><strong>Evaluation:<\/strong> Throughout the training, practical exercises with solutions will allow the trainee to assess their acquisition of knowledge. Trainees' learning outcomes will be assessed at the end of the training and a training certificate will be issued.<\/li>\n<\/ul>\n<hr \/>\n<h3 style=\"text-align: justify;\">PROGRAMME<\/h3>\n<h5 style=\"text-align: justify;\">The course will emphasise the basic knowledge required to understand the different techniques and potential applications. Practical work will demonstrate the advantages and limitations of the various systems.<\/h5>\n<ul>\n<li>Flow cytometry: principles, components of a cytometer, fluorescence and imaging flow cytometry<\/li>\n<li>Applications in flow cytometry: cell cycle, apoptosis, phenotyping, cell interaction, imaging flow cytometry, cytokine assay, metabolic probes, cell sorting<\/li>\n<li>Microscopy: the different types of light microscopy (bright-field, phase contrast, fluorescence?)<\/li>\n<li>Fluorescence microscopy: labelling, biosensors and applications<\/li>\n<li>The sample under the microscope: light\/sample interaction and sample preparation<\/li>\n<li>The different types of fluorescence microscopes: performance, advantages and disadvantages<\/li>\n<li>Overview of sample preparation methods for transmission electron microscopy (TEM)<\/li>\n<li>Understand the basic principles of transmission electron microscopes<\/li>\n<li>Principles of scanning electron microscopes (SEM)<\/li>\n<li>SEM sample preparation<\/li>\n<li>Overview of data analysis<\/li>\n<li>Application to image analysis<\/li>\n<\/ul>\n<hr \/>\n<h3 style=\"text-align: justify;\">EQUIPMENT &amp; PERSONNEL<\/h3>\n<ul>\n<li style=\"text-align: justify;\">Widefield, confocal, multiphoton and light-sheet microscopes, cytometers analysers, sorters, cytometers imaging, transmission electron microscopes and scanning electron microscopes,<\/li>\n<li>Engineers from the following research institutes: Agrobiosciences, Interactions and Biodiversity Research Federation; Biology and Biotechnology for Health Research Federation,<\/li>\n<li>The trainee is advised to bring their own laptop.<\/li>\n<\/ul>\n<hr \/>\n<p><img decoding=\"async\" class=\"alignright size-full wp-image-4224\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2016\/07\/CNRSformentrep.jpg\" alt=\"CNRS Corporate Training\" width=\"296\" height=\"77\" \/><\/p>\n<blockquote>\n<h3 style=\"text-align: justify;\">Contact and registration<\/h3>\n<ul>\n<li style=\"text-align: justify;\">For further information, contact: <a href=\"mailto:jacques.rouquette@cnrs.fr\"><strong>Jacques Rouquette<\/strong><\/a><\/li>\n<li style=\"text-align: justify;\"><a href=\"https:\/\/cnrsformation.cnrs.fr\/index.php?c=stage&amp;stage=24309\" target=\"_blank\" rel=\"nofollow noopener\"><strong>Applications on the CNRS website<\/strong><\/a><\/li>\n<\/ul>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"<p>Alternance de cours th\u00e9oriques (50 %) et de travaux pratiques (50 %), cette formation intensive est destin\u00e9 au personnel des entreprises d\u00e9sireuses d&rsquo;am\u00e9liorer leurs connaissances et comp\u00e9tences concernant l&rsquo;acquisition et l&rsquo;interpr\u00e9tation d&rsquo;images sur diff\u00e9rents types de microscopes et cytom\u00e8tres. \u00c9v\u00e9nement intensif se d\u00e9roulant sur 4 jours et demi, il m\u00ealera cours (limit\u00e9 \u00e0 10 stagiaires), [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7122,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[205],"tags":[],"class_list":["post-7820","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-formation"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Formation : Microscopies photonique et \u00e9lectronique, cytom\u00e9trie et analyse des donn\u00e9es -<\/title>\n<meta name=\"description\" content=\"Derni\u00e8res places disponibles pour la formation portant sur les bases et nouveaut\u00e9s de la microscopie de fluorescence.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/training-in-photonic-and-electronic-microscopy-cytometry-data-analysis\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Formation : Microscopies photonique et \u00e9lectronique, cytom\u00e9trie et analyse des donn\u00e9es -\" \/>\n<meta property=\"og:description\" content=\"Derni\u00e8res places disponibles pour la formation portant sur les bases et nouveaut\u00e9s de la microscopie de fluorescence.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/training-in-photonic-and-electronic-microscopy-cytometry-data-analysis\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2024-03-18T12:52:51+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-20T14:54:57+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"400\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" 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\u00e9lectronique, cytom\u00e9trie et analyse des donn\u00e9es\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\\\/#primaryimage\"},\"image\":[\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1-150x100.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1-300x200.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\"],\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\",\"datePublished\":\"2024-03-18T12:52:51+02:00\",\"dateModified\":\"2025-11-20T14:54:57+02:00\",\"description\":\"Alternance de cours th\u00e9oriques (50 %) et de travaux pratiques (50 %), cette formation intensive est destin\u00e9 au personnel des entreprises d\u00e9sireuses d'am\u00e9liorer leurs connaissances et comp\u00e9tences concernant l'acquisition et l'interpr\u00e9tation d'images sur diff\u00e9rents types de microscopes et cytom\u00e8tres. \u00c9v\u00e9nement intensif se d\u00e9roulant sur 4 jours et demi, il m\u00ealera cours (limit\u00e9 \u00e0 10 stagiaires), travaux pratiques (sous-groupes de 4 personnes maximum avec un \u00e0 deux intervenants) et sera maill\u00e9 d'exercices visant \u00e0 accompagner les stagiaires dans leur progression.\\n \\nOBJECTIFS\\n \\tAcqu\u00e9rir des bases th\u00e9oriques en microscopie photonique et \u00e9lectronique, en cytom\u00e9trie et analyse des donn\u00e9es,\\n \\tConna\u00eetre les applications possibles pour chaque technologie,\\n \\tComprendre l'aspect pratique de ces m\u00e9thodes\\nPUBLIC &amp; MODALIT\u00c9S\\n \\tPublic : Chercheurs, ing\u00e9nieurs et techniciens souhaitant d\u00e9couvrir l'ensemble de ces technologies\\n \\tPr\u00e9requis : Aucun, si ce n'est un int\u00e9r\u00eat clairement affich\u00e9 pour l'imagerie optique du vivant\\n \\tEffectifs : 10 stagiaires maximum, TP en sous-groupes de 4 stagiaires maximum avec 1 intervenant par sous-groupe\\n \\t\u00c9valuation : Tout au long de la formation, des exercices corrig\u00e9s permettront au stagiaire d'\u00e9valuer son acquisition des connaissances. Les acquis des stagiaires seront \u00e9valu\u00e9s \u00e0 l\u2019issue de la formation et attestation de formation sera d\u00e9livr\u00e9e.\\nPROGRAMME\\nLe cours insistera sur les connaissances de base requises pour comprendre les diff\u00e9rentes techniques et les applications possibles. Des travaux pratiques pr\u00e9senteront les avantages et limites des diff\u00e9rents syst\u00e8mes.\\n \\tLa cytom\u00e9trie : principes, composants d'un cytom\u00e8tre, la fluorescence et la cytom\u00e9trie en image\\n \\tApplications en cytom\u00e9trie : cycle cellulaire, apoptose, ph\u00e9notypage, interaction cellulaire, cytom\u00e9trie en image, dosage de cytokines, sondes m\u00e9taboliques, tri cellulaire\\n \\tLa microscopie : les diff\u00e9rents types de microscopie photonique (fond clair, contraste de phase, fluorescence?)\\n \\tLa microscopie de fluorescence : marquage, biosenseurs et applications\\n \\tL'\u00e9chantillon sous le microscope : interaction lumi\u00e8re \\\/ \u00e9chantillon et pr\u00e9paration d'\u00e9chantillon\\n \\tLes diff\u00e9rents types de microscopes de fluorescence : performances, avantages et inconv\u00e9nients\\n \\tPr\u00e9sentation des m\u00e9thodes pour la pr\u00e9paration d'\u00e9chantillons en microscopie \u00e9lectronique en transmission (MET)\\n \\tComprendre les principes de base des microscopes \u00e9lectroniques en transmission\\n \\tPrincipes des microscopes \u00e9lectroniques \u00e0 balayage (MEB)\\n \\tPr\u00e9paration des \u00e9chantillons en MEB\\n \\tG\u00e9n\u00e9ralit\u00e9s sur l'analyse de donn\u00e9es\\n \\tApplication \u00e0 l'analyse d'images\\n\u00c9QUIPEMENTS &amp; INTERVENANTS\\n \\tMicroscopes champ large, confocaux, multiphotons, \u00e0 feuille de lumi\u00e8re, cytom\u00e8tres analyseurs, trieurs, cytom\u00e8tres en image, microscopes \u00e9lectroniques \u00e0 transmission, microscopes \u00e9lectroniques \u00e0 balayage,\\n \\tIng\u00e9nieurs des instituts de recherche suivants : F\u00e9d\u00e9ration de recherche agrobiosciences, interactions et biodiversit\u00e9 ; F\u00e9d\u00e9ration de recherche biologie et biotechnologie pour la sant\u00e9,\\n \\tIl est recommand\u00e9 au stagiaire de se munir de son propre ordinateur portable.\\nContact et inscription\\n \\tPour plus d'informations, contact : Jacques Rouquette\\n \\tInscriptions sur le site du CNRS\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\\\/\"]}],\"@context\":\"https:\\\/\\\/schema.org\",\"startDate\":\"2024-06-03\",\"endDate\":\"2024-06-07\",\"eventStatus\":\"EventScheduled\",\"eventAttendanceMode\":\"OfflineEventAttendanceMode\",\"location\":{\"@type\":\"place\",\"name\":\"Multi-sites - r\u00e9gion toulousaine\",\"address\":\"Multi-sites - r\u00e9gion 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minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/#article","isPartOf":{"@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/"},"author":{"name":"gesteve","@id":"https:\/\/trigenotoul.com\/#\/schema\/person\/71018a7e00cf92ab28949e5339a08591"},"headline":"Formation : Microscopies photonique et \u00e9lectronique, cytom\u00e9trie et analyse des 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analysis","isPartOf":{"@id":"https:\/\/trigenotoul.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/#primaryimage"},"image":["https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1-150x100.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1-300x200.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg"],"thumbnailUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","datePublished":"2024-03-18T12:52:51+02:00","dateModified":"2025-11-20T14:54:57+02:00","description":"A combination of theory-based sessions (50 %) and practical sessions (50 %), this intensive course is aimed at staff from companies wishing to improve their knowledge and skills regarding the acquisition and interpretation of images using various types of microscopes and cytometers. This intensive four-and-a-half-day event will combine lectures (limited to 10 participants), practical sessions (in sub-groups of up to four people with one or two instructors) and will be interspersed with exercises designed to support participants\u2019 progress.\n \nOBJECTIVES\n     To acquire a theoretical foundation in photonic and electron microscopy, cytometry and data analysis,\n     To understand the possible applications of each technology,\n \tTo understand the practical aspects of these methods\nTARGET AUDIENCE &amp; DETAILS\n     Target audience: Researchers, engineers and technicians wishing to explore all of these technologies\n     Prerequisites: None, other than a clear interest in optical imaging of living organisms\n \tGroup size: Maximum of 10 participants; practical sessions in sub-groups of up to 4 participants with 1 instructor per sub-group\n     Assessment: Throughout the course, marked exercises will enable participants to assess their acquisition of knowledge. Trainees\u2019 learning outcomes will be assessed at the end of the course and a certificate of completion will be issued.\nPROGRAMME\nThe course will focus on the basic knowledge required to understand the various techniques and their potential applications. Practical sessions will highlight the advantages and limitations of the different systems.\n \tCytometry: principles, components of a cytometer, fluorescence and imaging cytometry\n     Applications in cytometry: cell cycle, apoptosis, phenotyping, cell-cell interaction, imaging cytometry, cytokine assays, metabolic probes, cell sorting\n \tMicroscopy: the different types of photonic microscopy (brightfield, phase contrast, fluorescence, etc.)\n     Fluorescence microscopy: labelling, biosensors and applications\n     The sample under the microscope: light\u2013sample interaction and sample preparation\n \tThe different types of fluorescence microscopes: performance, advantages and disadvantages\n     Overview of methods for sample preparation in transmission electron microscopy (TEM)\n \tUnderstanding the basic principles of transmission electron microscopes\n     Principles of scanning electron microscopes (SEM)\n     Sample preparation for SEM\n     Overview of data analysis\n     Application to image analysis\nEQUIPMENT &amp; INSTRUCTORS\n     Wide-field, confocal, multiphoton and light-sheet microscopes; cytometers analysers and sorters; cytometers imaging systems; transmission electron microscopes; scanning electron microscopes;\n \tEngineers from the following research institutes: Agrobiosciences, Interactions and Biodiversity Research Federation; Biology and Biotechnology for Health Research Federation,\n     Trainees are advised to bring their own laptop.\nContact and registration\n     For further information, please contact: Jacques Rouquette\n     Register on the CNRS website","breadcrumb":{"@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/"]}],"@context":"https:\/\/schema.org","startDate":"2024-06-03","endDate":"2024-06-07","eventStatus":"EventScheduled","eventAttendanceMode":"OfflineEventAttendanceMode","location":{"@type":"place","name":"Multi-sites - r\u00e9gion toulousaine","address":"Multi-sites - r\u00e9gion toulousaine","geo":{"@type":"GeoCoordinates","latitude":"43.60555325489756","longitude":"1.4467250030677248"}},"offers":{"@type":"Offer"}},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/#primaryimage","url":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","contentUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","width":600,"height":400,"caption":"CNRS Formation"},{"@type":"BreadcrumbList","@id":"https:\/\/trigenotoul.com\/formation-microscopies-photonique-et-electronique-cytometrie-analyse-donnees\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/trigenotoul.com\/"},{"@type":"ListItem","position":2,"name":"Formation : Microscopies photonique et \u00e9lectronique, cytom\u00e9trie et analyse des donn\u00e9es"}]},{"@type":"WebSite","@id":"https:\/\/trigenotoul.com\/#website","url":"https:\/\/trigenotoul.com\/","name":"TRI-Genotoul","description":"Imaging, cytometry &amp; 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