{"id":7671,"date":"2023-09-11T14:06:09","date_gmt":"2023-09-11T12:06:09","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=7671"},"modified":"2025-11-20T15:55:10","modified_gmt":"2025-11-20T14:55:10","slug":"fluorescence-microscopy-fundamentals-and-new-developments","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/la-microscopie-de-fluorescence-bases-et-nouveautes\/","title":{"rendered":"Fluorescence microscopy: basics and new developments"},"content":{"rendered":"<h5><img decoding=\"async\" class=\"alignleft wp-image-7122\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg\" alt=\"CNRS Training\" width=\"201\" height=\"140\" \/>This intensive training course is aimed at academic or corporate staff wishing to improve their knowledge and skills regarding the acquisition and interpretation of images on different types of fluorescence microscopes. This 4.5-day intensive event will combine lectures (limited to 10 trainees) and practical work (in subgroups of a maximum of 4 people), and will be punctuated by exercises designed to support trainees in their progress.<\/h5>\n<p>&nbsp;<\/p>\n<blockquote style=\"max-width: 380px !important; float: right; font-size: 1.3em; line-height: 1.6em; margin: 40px 0 20px 40px; padding: 130px 20px 30px 20px; text-align: left !important; background-color: #81993C;\">\n<div style=\"text-align: center; margin-top: -250px; padding-top: 50px;\"><img decoding=\"async\" id=\"gui\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2018\/04\/guillemets.png\" alt=\"guillemets\" width=\"130\" height=\"130\" hspace=\"10\" \/><\/div>\n<p>Good balance between theory and practice (good timing in the afternoon for the practical sessions). The wide variety of trainers brings dynamism to the training and allows the different concepts to be approached from different angles for a better understanding.\u00bb<\/p>\n<hr \/>\n<p>Employee, TRANSGENE SA<\/p><\/blockquote>\n<h3 style=\"text-align: justify;\">OBJECTIVES<\/h3>\n<ul style=\"text-align: justify;\">\n<li>Acquire theoretical and practical foundations in fluorescence microscopy<\/li>\n<li>Understand the advantages and limitations of various image acquisition systems in microscopy<\/li>\n<li>Learn about new developments in fluorescence microscopy<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\">PUBLIC &amp; MODALITIES<\/h3>\n<ul>\n<li style=\"text-align: justify;\"><strong>Public<\/strong> Researchers, engineers, and technicians wishing to familiarise themselves with fluorescence imaging techniques<\/li>\n<li><strong>Prerequisite:<\/strong> None, except for a clearly stated interest in in vivo optical imaging<\/li>\n<li style=\"text-align: justify;\"><strong>Staff numbers:<\/strong> Maximum of 10 trainees, practical sessions in sub-groups of maximum 4 trainees with 1 instructor per sub-group<\/li>\n<li style=\"text-align: justify;\"><strong>Evaluation:<\/strong> Throughout the training, practical exercises with solutions will allow the trainee to assess their acquisition of knowledge. Trainees' learning outcomes will be assessed at the end of the training and a training certificate will be issued.<\/li>\n<\/ul>\n<hr \/>\n<h3 style=\"text-align: justify;\">PROGRAMME<\/h3>\n<h5 style=\"text-align: justify;\"><strong>Course (40 %) \u2013 <\/strong>They will cover the basic knowledge required to acquire images under the best possible conditions, depending on the methods used, the diversity of the samples likely to be imaged, and the precautions to be taken to avoid misinterpretation.<\/h5>\n<ul style=\"text-align: justify;\">\n<li>Brightfield and fluorescence microscopy: principle, description, important adjustments, image acquisition on a widefield system, CCD and C-MOS cameras<\/li>\n<li>Probes and fluorescent proteins<\/li>\n<li>Analysis of molecular interactions and movements: F-techniques (FRET, FLIM, FCS, FRAP, etc.)<\/li>\n<li>Single- and multi-photon confocal microscopy: principles and applications<\/li>\n<li>Image sampling, resolution and deconvolution<\/li>\n<li>3D imaging in light sheet microscopy<\/li>\n<li>Super-resolution techniques<\/li>\n<li>Sample preparation<\/li>\n<li>Intravital imaging and small animal imaging<\/li>\n<\/ul>\n<h5 style=\"text-align: justify;\"><strong>Practical work (60 %)<\/strong> \u2013 Carried out in small groups on various equipment at the Toulouse imaging platform (widefield, confocal, multiphoton, SPIM microscopes, etc.)<\/h5>\n<ul style=\"text-align: justify;\">\n<li>Advantages and limitations of the different systems<\/li>\n<li>Acquisition and analysis of images under the best conditions<\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><em>Possibility of carrying out the practical work, for educational purposes, on samples brought in by trainees<\/em><\/p>\n<hr \/>\n<h3 style=\"text-align: justify;\">EQUIPMENT &amp; PERSONNEL<\/h3>\n<ul>\n<li style=\"text-align: justify;\">Wide-field microscopes, confocal microscopes, multiphoton microscopes, CCD cameras, light sheet microscope and macroscope (SPIM), intravital microscope, whole-body fluorescence imaging system<\/li>\n<li style=\"text-align: justify;\">Researchers and engineers from the following research institutes: Agrobiosciences, Interactions and Biodiversity Research Federation; Toulouse Biomedical Research Federation (SFR BMT)<\/li>\n<\/ul>\n<hr \/>\n<p><img decoding=\"async\" class=\"alignright size-full wp-image-4224\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2016\/07\/CNRSformentrep.jpg\" alt=\"CNRS Corporate Training\" width=\"296\" height=\"77\" \/><\/p>\n<blockquote>\n<h3 style=\"text-align: justify;\">Contact and registration<\/h3>\n<ul>\n<li style=\"text-align: justify;\">For further information, contact: <a href=\"mailto:jacques.rouquette@cnrs.fr\"><strong>Jacques Rouquette<\/strong><\/a><\/li>\n<li style=\"text-align: justify;\"><a href=\"https:\/\/cnrsformation.cnrs.fr\/microscopie-de-fluorescence-bases-et-nouveautes?axe=184\" target=\"_blank\" rel=\"nofollow noopener\"><strong>Applications on the CNRS website<\/strong><\/a><\/li>\n<\/ul>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"<p>Cette formation intensive est destin\u00e9 au personnel acad\u00e9mique ou entreprise d\u00e9sireux d&rsquo;am\u00e9liorer les connaissances et comp\u00e9tences concernant l&rsquo;acquisition et l&rsquo;interpr\u00e9tation d&rsquo;images sur diff\u00e9rents types de microscopes \u00e0 fluorescence. \u00c9v\u00e9nement intensif se d\u00e9roulant sur 4 jours et demi, il m\u00ealera cours (limit\u00e9 \u00e0 10 stagiaires) et travaux pratiques (sous-groupes de 4 personnes maximum), il sera maill\u00e9 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7122,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[205],"tags":[],"class_list":["post-7671","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-formation"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>La microscopie de fluorescence : bases et nouveaut\u00e9s -<\/title>\n<meta name=\"description\" content=\"Derni\u00e8res places disponibles pour la formation portant sur les bases et nouveaut\u00e9s de la microscopie de fluorescence.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/fluorescence-microscopy-fundamentals-and-new-developments\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"La microscopie de fluorescence : bases et nouveaut\u00e9s -\" \/>\n<meta property=\"og:description\" content=\"Derni\u00e8res places disponibles pour la formation portant sur les bases et nouveaut\u00e9s de la microscopie de fluorescence.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/fluorescence-microscopy-fundamentals-and-new-developments\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2023-09-11T12:06:09+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-20T14:55:10+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"400\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"La microscopie de fluorescence : bases et nouveaut\u00e9s\",\"datePublished\":\"2023-09-11T12:06:09+00:00\",\"dateModified\":\"2025-11-20T14:55:10+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/\"},\"wordCount\":537,\"publisher\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\",\"articleSection\":[\"Formation\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"event\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/\",\"name\":\"La microscopie de fluorescence : bases et nouveaut\u00e9s\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/la-microscopie-de-fluorescence-bases-et-nouveautes\\\/#primaryimage\"},\"image\":[\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1-150x100.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1-300x200.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\"],\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/CNRSformation-1.jpg\",\"datePublished\":\"2023-09-11T12:06:09+02:00\",\"dateModified\":\"2025-11-20T14:55:10+02:00\",\"description\":\"Cette formation intensive est destin\u00e9 au personnel acad\u00e9mique ou entreprise d\u00e9sireux d'am\u00e9liorer les connaissances et comp\u00e9tences concernant l'acquisition et l'interpr\u00e9tation d'images sur diff\u00e9rents types de microscopes \u00e0 fluorescence. \u00c9v\u00e9nement intensif se d\u00e9roulant sur 4 jours et demi, il m\u00ealera cours (limit\u00e9 \u00e0 10 stagiaires) et travaux pratiques (sous-groupes de 4 personnes maximum), il sera maill\u00e9 d'exercices visant \u00e0 accompagner les stagiaires dans leur progression.\\n \\nBon \u00e9quilibre entre th\u00e9orie et pratique (bon timing l'apr\u00e8s-midi pour les TP). La grande vari\u00e9t\u00e9 des intervenants donne du dynamisme \u00e0 la formation et permet d'aborder les diff\u00e9rentes notions sous des angles diff\u00e9rents pour une meilleure compr\u00e9hension\\\"\\nSalari\u00e9e, TRANSGENE SA\\nOBJECTIFS\\n \\tAcqu\u00e9rir des bases th\u00e9oriques et pratiques sur la microscopie de fluorescence\\n \\tConna\u00eetre les avantages et les limites des divers syst\u00e8mes d'acquisition d'images en microscopie\\n \\tS'initier aux nouveaux d\u00e9veloppements en microscopie de fluorescence\\nPUBLIC &amp; MODALIT\u00c9S\\n \\tPublic : Chercheurs, ing\u00e9nieurs, techniciens d\u00e9sireux de s'initier aux techniques d'imagerie en fluorescence\\n \\tPr\u00e9requis : Aucun, si ce n'est un int\u00e9r\u00eat clairement affich\u00e9 pour l'imagerie optique du vivant\\n \\tEffectifs : 10 stagiaires maximum, TP en sous-groupes de 4 stagiaires maximum avec 1 intervenant par sous-groupe\\n \\t\u00c9valuation : Tout au long de la formation, des exercices corrig\u00e9s permettront au stagiaire d'\u00e9valuer son acquisition des connaissances. Les acquis des stagiaires seront \u00e9valu\u00e9s \u00e0 l\u2019issue de la formation et attestation de formation sera d\u00e9livr\u00e9e.\\nPROGRAMME\\nCours (40 %) - Ils porteront sur les connaissances de base requises pour acqu\u00e9rir des images dans les meilleures conditions possibles, en fonction des m\u00e9thodes utilis\u00e9es, sur la diversit\u00e9 des \u00e9chantillons susceptibles d'\u00eatre imag\u00e9s et sur les pr\u00e9cautions \u00e0 prendre pour \u00e9viter les erreurs d'interpr\u00e9tation.\\n \\tLe microscope en fond clair et \u00e0 fluorescence : principe, description, r\u00e9glages importants, acquisition d'images sur syst\u00e8me champ large, les cam\u00e9ras CCD et C-MOS\\n \\tLes sondes et les prot\u00e9ines fluorescentes\\n \\tAnalyse des interactions et mouvements mol\u00e9culaires : F-Techniques (FRET, FLIM, FCS, FRAP...)\\n \\tLa microscopie confocale mono- et multiphotonique : principes et applications\\n \\t\u00c9chantillonnage, r\u00e9solution et d\u00e9convolution des images\\n \\tImagerie 3D en microscopie \u00e0 feuille de lumi\u00e8re\\n \\tLes techniques de super-r\u00e9solution\\n \\tLa pr\u00e9paration des \u00e9chantillons\\n \\tL'imagerie intravitale et l'imagerie du petit animal\\nTravaux pratiques (60 %) - R\u00e9alis\u00e9s en petits groupes sur diff\u00e9rents appareillages de la plateforme d'imagerie toulousaine (microscopes champ large, confocal, multiphoton, SPIM...)\\n \\tAvantages et limites des diff\u00e9rents syst\u00e8mes\\n \\tAcquisition et analyse des images dans les meilleures conditions\\nPossibilit\u00e9 de faire les TP, \u00e0 des fins p\u00e9dagogiques, sur des \u00e9chantillons apport\u00e9s par les stagiaires\\n\u00c9QUIPEMENTS &amp; INTERVENANTS\\n \\tMicroscopes champ large, microscopes confocaux, microscope multiphotons, cam\u00e9ras CCD, microscope et macroscope \u00e0 feuille de lumi\u00e8re (SPIM), microscope intravital, syst\u00e8me d'acquisition de fluorescence corps entier\\n \\tChercheurs et ing\u00e9nieurs des instituts de recherche suivants : F\u00e9d\u00e9ration de recherche agrobiosciences, interactions et biodiversit\u00e9 ; F\u00e9d\u00e9ration de recherche biom\u00e9dicale de Toulouse (SFR BMT)\\nContact et inscription\\n \\tPour plus d'informations, contact : Jacques Rouquette\\n \\tInscriptions sur le site du 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developments","isPartOf":{"@id":"https:\/\/trigenotoul.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/trigenotoul.com\/la-microscopie-de-fluorescence-bases-et-nouveautes\/#primaryimage"},"image":["https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1-150x100.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1-300x200.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg"],"thumbnailUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","datePublished":"2023-09-11T12:06:09+02:00","dateModified":"2025-11-20T14:55:10+02:00","description":"This intensive course is aimed at academic and corporate staff wishing to improve their knowledge and skills in acquiring and interpreting images using various types of fluorescence microscopes. This intensive four-and-a-half-day event will combine lectures (limited to 10 participants) with practical sessions (in groups of no more than four people), and will feature a series of exercises designed to support participants\" progress.\n \nA good balance between theory and practice (well-timed practical sessions in the afternoon). The wide variety of speakers brings the course to life and allows different concepts to be approached from different angles, leading to a better understanding&quot;\nEmployee, TRANSGENE SA\nOBJECTIVES\n     To acquire a theoretical and practical grounding in fluorescence microscopy\n     To understand the advantages and limitations of various image acquisition systems in microscopy\n     To gain an introduction to new developments in fluorescence microscopy\nTARGET AUDIENCE &amp; FORMAT\n     Target audience: Researchers, engineers and technicians wishing to learn about fluorescence imaging techniques\n     Prerequisites: None, other than a clear interest in optical imaging of living organisms\n \tGroup size: Maximum 10 participants; practical sessions in sub-groups of up to 4 participants with 1 instructor per sub-group\n     Assessment: Throughout the course, marked exercises will enable participants to assess their acquisition of knowledge. Trainees\u2019 learning outcomes will be assessed at the end of the course and a certificate of completion will be issued.\nPROGRAMME\nLectures (40 %) \u2013 These will cover the basic knowledge required to acquire images under the best possible conditions, depending on the methods used, the variety of samples likely to be imaged, and the precautions to be taken to avoid misinterpretation.\n \tBrightfield and fluorescence microscopy: principles, description, key settings, image acquisition using a wide-field system, CCD and CMOS cameras\n \tFluorescent probes and proteins\n     Analysis of molecular interactions and movements: F-techniques (FRET, FLIM, FCS, FRAP, etc.)\n \tSingle- and multi-photon confocal microscopy: principles and applications\n     Sampling, resolution and image deconvolution\n     3D imaging in light-sheet microscopy\n     Super-resolution techniques\n     Sample preparation\n \tIn vivo imaging and small-animal imaging\nPractical sessions (60 %) \u2013 Conducted in small groups using various instruments at the Toulouse imaging platform (wide-field, confocal, multiphoton and SPIM microscopes, etc.)\n \tAdvantages and limitations of the various systems\n     Image acquisition and analysis under optimal conditions\nOption to carry out practical sessions, for educational purposes, using samples provided by the trainees\nEQUIPMENT &amp; INSTRUCTORS\n     Wide-field microscopes, confocal microscopes, multiphoton microscopes, CCD cameras, sheet-light microscope and macroscope (SPIM), intravital microscope, whole-body fluorescence imaging system\n \tResearchers and engineers from the following research institutes: Agroscience, Interactions and Biodiversity Research Federation; Toulouse Biomedical Research Federation (SFR BMT)\nContact and registration\n     For further information, please contact: Jacques Rouquette\n     Register on the CNRS website","breadcrumb":{"@id":"https:\/\/trigenotoul.com\/la-microscopie-de-fluorescence-bases-et-nouveautes\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/trigenotoul.com\/la-microscopie-de-fluorescence-bases-et-nouveautes\/"]}],"@context":"https:\/\/schema.org","startDate":"2023-10-09","endDate":"2023-10-13","eventStatus":"EventScheduled","eventAttendanceMode":"OfflineEventAttendanceMode","location":{"@type":"place","name":"Multisite","address":"Multisite","geo":{"@type":"GeoCoordinates","latitude":"43.599998653321165","longitude":"1.434383999034384"}},"offers":{"@type":"Offer"}},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/trigenotoul.com\/la-microscopie-de-fluorescence-bases-et-nouveautes\/#primaryimage","url":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","contentUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/03\/CNRSformation-1.jpg","width":600,"height":400,"caption":"CNRS Formation"},{"@type":"BreadcrumbList","@id":"https:\/\/trigenotoul.com\/la-microscopie-de-fluorescence-bases-et-nouveautes\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/trigenotoul.com\/"},{"@type":"ListItem","position":2,"name":"La microscopie de fluorescence : bases et nouveaut\u00e9s"}]},{"@type":"WebSite","@id":"https:\/\/trigenotoul.com\/#website","url":"https:\/\/trigenotoul.com\/","name":"TRI-Genotoul","description":"Imaging, cytometry &amp; 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