{"id":7613,"date":"2023-06-22T11:55:28","date_gmt":"2023-06-22T09:55:28","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=7613"},"modified":"2023-06-22T11:55:28","modified_gmt":"2023-06-22T09:55:28","slug":"un-nouveau-plateau-au-sein-de-tri","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/un-nouveau-plateau-au-sein-de-tri\/","title":{"rendered":"A new platform within TRI"},"content":{"rendered":"<h5>La plateforme Organo\u00efdes de l&rsquo;IRSD (Institut de Recherche en Sant\u00e9 Digestive), dirig\u00e9 par David Sagnat et Nathalie Vergnolle, sur le site de Purpan vient renforcer le r\u00e9seau TRI. Cette plateforme cr\u00e9e in vitro et en trois dimensions des mini-organes \u00e9pith\u00e9liaux polaris\u00e9s, \u00e9galement appel\u00e9s organo\u00efdes, \u00e0 partir de cellules souches isol\u00e9es de biopsies ou de r\u00e9sections de patients humains ou de souris.<\/h5>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">Nous sommes sp\u00e9cialis\u00e9s dans les organo\u00efdes de c\u00f4lon de patients sains et inflammatoires (maladie de Crohn et rectocolite h\u00e9morragique) et avons constitu\u00e9 une biobanque d\u2019organo\u00efdes d\u2019environ 250 patients sains et inflammatoires que nous continuons d\u2019incr\u00e9menter explique David Sagnat, responsable op\u00e9rationnel de la plateforme. <img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-7632 alignleft\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1024x814.jpg\" alt=\"\" width=\"713\" height=\"567\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1024x814.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-300x239.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-768x611.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1536x1221.jpg 1536w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-440x350.jpg 440w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-150x119.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1320x1049.jpg 1320w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1.jpg 1883w\" sizes=\"(max-width: 713px) 100vw, 713px\" \/>La plateforme poss\u00e8de \u00e9galement une machine de criblage \u00e0 haut contenu qui nous permet d\u2019acqu\u00e9rir ce genre de structures : l&rsquo;Opera Phenix de Perkin Elmer. Elle permet d&rsquo;analyser la morphologie (taille, \u00e9paisseur, p\u00e9rim\u00e8tre, surface) de nombreuses structures par plaque enti\u00e8re (de 6 \u00e0 384 puits, lames, IBIDI&#8230;) durant une m\u00eame acquisition et ce de mani\u00e8re compl\u00e8tement automatis\u00e9e.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<blockquote>\n<h5>Nous utilisons actuellement cette technologie pour des structures 3D (organo\u00efdes) mais bien \u00e9videmment aussi pour des structures 2D (neurones, lymphocytes, fibroblastes, \u2026) pour de nombreux utilisateurs et projets.<\/h5>\n<h5>Nathalie Vergnolle<\/h5>\n<\/blockquote>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" class=\"alignleft wp-image-7630\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1.jpg\" alt=\"\" width=\"600\" height=\"450\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1.jpg 1920w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-300x225.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1024x767.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-768x576.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1536x1151.jpg 1536w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-467x350.jpg 467w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-150x112.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1320x989.jpg 1320w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/>Actuellement, la plateforme organo\u00efdes est compos\u00e9e de deux membres : Nathalie Vergnolle, responsable scientifique et directrice de l&rsquo;IRSD et David Sagnat, responsable op\u00e9rationnel. Elle se verra renforcer d\u00e8s Septembre 2023, par Astrid Canivet. D\u00e9j\u00e0 famili\u00e8re de TRI-Genotoul, elle deviendra la personne d\u00e9di\u00e9e pour l\u2019accompagnement des utilisateurs sur l\u2019Op\u00e9ra Phenix pour la formation, l\u2019acquisition et l\u2019analyse.<\/p>\n<blockquote><p>&nbsp;<\/p>\n<h4>Expertises<\/h4>\n<ul>\n<li>Suivi des cultures 3D (imagerie), immunofluorescence sur organo\u00efdes<\/li>\n<li>Utilisation d&rsquo;un appareil d&rsquo;imagerie de criblage \u00e0 haut contenu (Opera Phenix &#8211; Perkin Elmer)<\/li>\n<li>Formation \u00e0 l\u2019utilisation de l\u2019appareil de criblage \u00e0 haut contenu<\/li>\n<\/ul>\n<\/blockquote>\n<hr \/>\n<blockquote><p><strong>Contact &amp; r\u00e9servation : <\/strong><a href=\"mailto:david.sagnat@inserm.fr?subject=TRI\"><strong>David Sagnat<\/strong><\/a><\/p>\n<hr \/>\n<h4>Publications<\/h4>\n<ul>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29959891\">Thrombin\u00a0<strong>modifies<\/strong>\u00a0<strong>growth<\/strong>,\u00a0<strong>proliferation<\/strong>\u00a0and\u00a0<strong>apoptosis<\/strong>\u00a0of\u00a0<strong>human<\/strong>\u00a0<strong>colon<\/strong>\u00a0<strong>organoids<\/strong>: a\u00a0<strong>protease-activated<\/strong>\u00a0<strong>receptor<\/strong>\u00a0<strong>1<\/strong>&#8211; and\u00a0<strong>protease-activated<\/strong>\u00a0<strong>receptor<\/strong>\u00a0<strong>4-dependent<\/strong>\u00a0<strong>mechanism<\/strong>.<\/a><br \/>\nS\u00e9bert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A, Vergnolle N.Br J Pharmacol. 2018 Sep;175(18):3656-3668. doi: 10.1111\/bph.14430. Epub 2018 Aug 7. &#8211; PMID: 29959891<\/li>\n<li>Patent: Urosphere &#8211; organo\u00efdes de vessie &#8211; Brevet n\u00b0 19290013.2 : Method for differenciating epithelial stem cells (Philippe Lluel, Xavier Game, Nathalie Vergnolle)<\/li>\n<\/ul>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"<p>La plateforme Organo\u00efdes de l&rsquo;IRSD (Institut de Recherche en Sant\u00e9 Digestive), dirig\u00e9 par David Sagnat et Nathalie Vergnolle, sur le site de Purpan vient renforcer le r\u00e9seau TRI. Cette plateforme cr\u00e9e in vitro et en trois dimensions des mini-organes \u00e9pith\u00e9liaux polaris\u00e9s, \u00e9galement appel\u00e9s organo\u00efdes, \u00e0 partir de cellules souches isol\u00e9es de biopsies ou de r\u00e9sections [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7631,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7613","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Un nouveau plateau au sein de TRI - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/un-nouveau-plateau-au-sein-de-tri\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Un nouveau plateau au sein de TRI - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"La plateforme Organo\u00efdes de l&rsquo;IRSD (Institut de Recherche en Sant\u00e9 Digestive), dirig\u00e9 par David Sagnat et Nathalie Vergnolle, sur le site de Purpan vient renforcer le r\u00e9seau TRI. 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