{"id":7613,"date":"2023-06-22T11:55:28","date_gmt":"2023-06-22T09:55:28","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=7613"},"modified":"2023-06-22T11:55:28","modified_gmt":"2023-06-22T09:55:28","slug":"a-new-sorting-platform","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/un-nouveau-plateau-au-sein-de-tri\/","title":{"rendered":"A new platform within TRI"},"content":{"rendered":"<h5>The Organoids platform of the IRSD (Digestive Health Research Institute), directed by David Sagnat and Nathalie Vergnolle, located at the Purpan site, reinforces the TRI network. This platform creates in vitro and in three dimensions polarised epithelial mini-organs, also known as organoids, from stem cells isolated from biopsies or resections from human patients or mice.<\/h5>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\">We specialise in colon organoids from healthy and inflammatory patients (Crohn's disease and ulcerative colitis) and have built up an organoid biobank of around 250 healthy and inflammatory patients, which we are continuing to grow, explains David Sagnat, operational manager of the platform. <img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-7632 alignleft\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1024x814.jpg\" alt=\"\" width=\"713\" height=\"567\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1024x814.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-300x239.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-768x611.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1536x1221.jpg 1536w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-440x350.jpg 440w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-150x119.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1-1320x1049.jpg 1320w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD1b-1.jpg 1883w\" sizes=\"(max-width: 713px) 100vw, 713px\" \/>The platform also has a high-content screening system that allows us to acquire these kinds of structures: the Opera Phenix from Perkin Elmer. It makes it possible to analyse the morphology (size, thickness, perimeter, surface area) of numerous structures per whole plate (from 6 to 384 wells, slides, IBIDI, etc.) during a single acquisition in a completely automated manner.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<blockquote>\n<h5>We are currently using this technology for 3D structures (organoids) but of course also for 2D structures (neurones, lymphocytes, fibroblasts, etc.) for numerous users and projects.<\/h5>\n<h5>Nathalie Vergnolle<\/h5>\n<\/blockquote>\n<p>&nbsp;<\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" class=\"alignleft wp-image-7630\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1.jpg\" alt=\"\" width=\"600\" height=\"450\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1.jpg 1920w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-300x225.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1024x767.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-768x576.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1536x1151.jpg 1536w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-467x350.jpg 467w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-150x112.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2023\/06\/IRSD3b-1-1320x989.jpg 1320w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/>Currently, the organoids platform is made up of two members: Nathalie Vergnolle, scientific lead and director of the IRSD, and David Sagnat, operational lead. It will be strengthened from September 2023 by Astrid Canivet. Already familiar with TRI-Genotoul, she will become the dedicated person for supporting users on the Opera Phenix for training, acquisition and analysis.<\/p>\n<blockquote><p>&nbsp;<\/p>\n<h4>Expertise<\/h4>\n<ul>\n<li>3D culture monitoring (imaging), immunofluorescence on organoids<\/li>\n<li>Use of a high-content screening imaging system (Opera Phenix \u2013 Perkin Elmer)<\/li>\n<li>Training on the use of the high-content screening device<\/li>\n<\/ul>\n<\/blockquote>\n<hr \/>\n<blockquote><p><strong>Contact &amp; booking: <\/strong><a href=\"mailto:david.sagnat@inserm.fr?subject=TRI\"><strong>David Sagnat<\/strong><\/a><\/p>\n<hr \/>\n<h4>Publications<\/h4>\n<ul>\n<li><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29959891\">Thrombin\u00a0<strong>modifies<\/strong>\u00a0<strong>growth<\/strong>,\u00a0<strong>proliferation<\/strong>\u00a0and\u00a0<strong>apoptosis<\/strong>\u00a0of\u00a0<strong>human<\/strong>\u00a0<strong>colon<\/strong>\u00a0<strong>organoids<\/strong>: a\u00a0<strong>protease-activated<\/strong>\u00a0<strong>receiver<\/strong>\u00a0<strong>1<\/strong>\u2013 and\u00a0<strong>protease-activated<\/strong>\u00a0<strong>receiver<\/strong>\u00a0<strong>4-dependent<\/strong>\u00a0<strong>mechanism<\/strong>.<\/a><br \/>\nS\u00e9bert M, Denadai-Souza A, Quaranta M, Racaud-Sultan C, Chabot S, Lluel P, Monjotin N, Alric L, Portier G, Kirzin S, Bonnet D, Ferrand A, Vergnolle N.Br J Pharmacol. 2018 Sep;175(18):3656-3668. doi: 10.1111\/bph.14430. Epub 2018 Aug 7. \u2013 PMID: 29959891<\/li>\n<li>Patent: Urosphere \u2013 bladder organoids \u2013 Patent No. 19290013.2: Method for differentiating epithelial stem cells (Philippe Lluel, Xavier Game, Nathalie Vergnolle)<\/li>\n<\/ul>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"<p>La plateforme Organo\u00efdes de l&rsquo;IRSD (Institut de Recherche en Sant\u00e9 Digestive), dirig\u00e9 par David Sagnat et Nathalie Vergnolle, sur le site de Purpan vient renforcer le r\u00e9seau TRI. Cette plateforme cr\u00e9e in vitro et en trois dimensions des mini-organes \u00e9pith\u00e9liaux polaris\u00e9s, \u00e9galement appel\u00e9s organo\u00efdes, \u00e0 partir de cellules souches isol\u00e9es de biopsies ou de r\u00e9sections [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":7631,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7613","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Un nouveau plateau au sein de TRI - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/a-new-sorting-platform\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Un nouveau plateau au sein de TRI - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"La plateforme Organo\u00efdes de l&rsquo;IRSD (Institut de Recherche en Sant\u00e9 Digestive), dirig\u00e9 par David Sagnat et Nathalie Vergnolle, sur le site de Purpan vient renforcer le r\u00e9seau TRI. 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