{"id":6972,"date":"2022-02-17T15:22:18","date_gmt":"2022-02-17T14:22:18","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=6972"},"modified":"2022-02-17T15:22:18","modified_gmt":"2022-02-17T14:22:18","slug":"latest-generation-cryo-em-at-the-meti","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/cryo-em-derniere-generation-au-meti\/","title":{"rendered":"State-of-the-art cryo-EM at METi"},"content":{"rendered":"<h5>The METi platform is now home to a fully automated cryo-electron microscope (Talos Arctica, ThermoFisher Scientific) equipped with a direct electron detection camera coupled to an energy filter (K2 summit BioQuantum, Gatan). This transmission electron microscope, dedicated to the high-resolution\/high-throughput observation of biological and organic samples frozen in amorphous ice, is fully operational.<\/h5>\n<hr \/>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignleft wp-image-6974\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica2-1.jpg\" alt=\"Talos Arctica\" width=\"300\" height=\"230\" \/> <img decoding=\"async\" class=\"alignleft wp-image-6973\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica1-1.jpg\" alt=\"Talos Arctica\" width=\"300\" height=\"276\" \/>One of the major applications of electron cryomicroscopy is the determination of the 3D structure of macromolecules at near-atomic resolution. This approach, which has made it possible to access the structure of coronaviruses for example, opens up very significant prospects in structural biology, pharmacology, virology, enzymology, etc. During the qualification tests, an initial series of image acquisitions led to the determination of the structure of apoferritin, a \u00abtest\u00bb enzyme well known to structural biologists, at a resolution of 2.35 \u00c5 in a few days.<\/p>\n<p>&nbsp;<\/p>\n<p>This Talos Arctica will also make it possible to study the three-dimensional structure of cells or other nano-objects, biological or otherwise, using cryo-electron tomography. With this new device, Toulouse is joining the currently very exclusive club of French sites equipped with high-throughput \/ high-resolution cryo-microscopes. Installed in the new 4R4 building of the <a href=\"https:\/\/trigenotoul.com\/en\/tri-genotoul\/our-platforms\/\">Centre for Integrative Biology<\/a>, the Toulouse Talos Arctica is open to projects from the local and national scientific community.<\/p>\n<figure id=\"attachment_6975\" aria-describedby=\"caption-attachment-6975\" style=\"width: 1364px\" class=\"wp-caption alignleft\"><img decoding=\"async\" class=\"size-full wp-image-6975\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1.jpg\" alt=\"Talos Artica imaging\" width=\"1364\" height=\"349\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1.jpg 1364w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-300x77.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-1024x262.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-768x197.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-900x230.jpg 900w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-150x38.jpg 150w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2022\/02\/talosartica3-1-1320x338.jpg 1320w\" sizes=\"(max-width: 1364px) 100vw, 1364px\" \/><figcaption id=\"caption-attachment-6975\" class=\"wp-caption-text\">Apoferritin molecules observed on the Toulouse Talos Arctica (right) and 3D structure of apoferritin resolved at 2.35 \u00c5 (miPlateau: global surface view; right: detail of the structure and derived atomic model).<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<hr \/>\n<blockquote>\n<h3>Contact and booking<\/h3>\n<ul>\n<li>Contact: <a href=\"mailto:stephanie.balor@utoulouse.fr\"><strong>St\u00e9phanie Balor<\/strong><\/a><\/li>\n<li><strong><a href=\"https:\/\/vm-tridb.toulouse.INRAe.fr\/\" target=\"_blank\" rel=\"noopener\">Online booking<\/a><\/strong><\/li>\n<\/ul>\n<\/blockquote>","protected":false},"excerpt":{"rendered":"<p>Le plateau du METi abrite d\u00e9sormais un cryo-microscope \u00e9lectronique (Talos Arctica, ThermoFisher Scientific) enti\u00e8rement automatis\u00e9 et \u00e9quip\u00e9 d\u2019une cam\u00e9ra \u00e0 d\u00e9tection directe d\u2019\u00e9lectrons coupl\u00e9e \u00e0 un filtre de perte d\u2019\u00e9nergie (K2 summit BioQuantum, Gatan). Ce microscope \u00e9lectronique en transmission, d\u00e9di\u00e9 \u00e0 l\u2019observation haute r\u00e9solution\/haut d\u00e9bit d\u2019\u00e9chantillons biologiques et organiques congel\u00e9s dans de la glace amorphe [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6974,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6972","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cryo-EM derni\u00e8re g\u00e9n\u00e9ration au METi - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/latest-generation-cryo-em-at-the-meti\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cryo-EM derni\u00e8re g\u00e9n\u00e9ration au METi - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Le plateau du METi abrite d\u00e9sormais un cryo-microscope \u00e9lectronique (Talos Arctica, ThermoFisher Scientific) enti\u00e8rement automatis\u00e9 et \u00e9quip\u00e9 d\u2019une cam\u00e9ra \u00e0 d\u00e9tection directe d\u2019\u00e9lectrons coupl\u00e9e \u00e0 un filtre de perte d\u2019\u00e9nergie (K2 summit BioQuantum, Gatan). 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