{"id":6775,"date":"2021-07-02T18:07:01","date_gmt":"2021-07-02T16:07:01","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=6775"},"modified":"2025-11-20T15:55:44","modified_gmt":"2025-11-20T14:55:44","slug":"electron-microscopy-with-scanning","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/formation-microscopie-electronique-a-balayage\/","title":{"rendered":"Scanning Electron Microscopy Training"},"content":{"rendered":"<figure id=\"attachment_6776\" aria-describedby=\"caption-attachment-6776\" style=\"width: 561px\" class=\"wp-caption alignleft\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-6776\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/montageformatipon-1.jpg\" alt=\"3D visualisation of gelosomes in a hydrogel by high-pressure cryo-fixation \" width=\"561\" height=\"180\" \/><figcaption id=\"caption-attachment-6776\" class=\"wp-caption-text\">3D visualisation of gelosomes in a hydrogel by high-pressure cryo-fixation<\/figcaption><\/figure>\n<h5 style=\"text-align: justify;\">This training will enable you to acquire the skills for sample preparation and for observation using scanning electron microscopy. This training is divided into two parts. The first part, theoretical, will cover the preparation protocols (critical point and high-pressure freezing) and the operation of a scanning electron microscope. The second part will be devoted to practical work on sample preparation and observation.<\/h5>\n<hr \/>\n<h4>Course (3 hours):<\/h4>\n<ul>\n<li>Preparation of samples<\/li>\n<li>Principle of the MEB and the acquisition of images<\/li>\n<li>Examples of application to the MEB<\/li>\n<\/ul>\n<h4>Practical Work (13h):<\/h4>\n<ul>\n<li>Preparation of biological samples using the critical point method<\/li>\n<li>Preparation of biological samples by high-pressure cryo-fixation<\/li>\n<li>Observation of samples in the MEB<\/li>\n<li>Observation of samples in the cryo-MEB<\/li>\n<\/ul>\n<p><img decoding=\"async\" class=\"size-full wp-image-4223 alignleft\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2016\/07\/bandeauformation.jpg\" alt=\"\" width=\"1200\" height=\"115\" \/><\/p>\n<blockquote>\n<h4><strong>Information and registration <\/strong><\/h4>\n<ul>\n<li><strong>Duration:<\/strong> 2 days<\/li>\n<li><strong>Public<\/strong> Technician, engineer, researcher in a public or private research laboratory.<\/li>\n<li><strong>Prerequisite:<\/strong> Knowledge of biology or chemistry.<\/li>\n<li><strong>Worktop <\/strong>CMEAB (UT3 \u2013 Rangueil \u2013 Toulouse) for the courses and the observation in the MEB. MeTi (UT3 \u2013 Rangueil \u2013 Toulouse) for the cryofixation.<\/li>\n<li><a href=\"mailto:bruno.payre@utoulouse.fr\"><strong>Information and registration with Bruno Payre<\/strong><\/a><\/li>\n<\/ul>\n<\/blockquote>\n<p><img decoding=\"async\" class=\"alignleft size-full wp-image-6787\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1.jpg\" alt=\"UT3, CMEAB, TRI-Genotoul\" width=\"1249\" height=\"160\" srcset=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1.jpg 1249w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1-300x38.jpg 300w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1-1024x131.jpg 1024w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1-768x98.jpg 768w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1-900x115.jpg 900w, https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/bandeaulogo-1-150x19.jpg 150w\" sizes=\"(max-width: 1249px) 100vw, 1249px\" \/><\/p>","protected":false},"excerpt":{"rendered":"<p>Cette formation vous permettra d\u2019acqu\u00e9rir le comp\u00e9tences pour la pr\u00e9paration des \u00e9chantillons et pour l\u2019observation en microscopie \u00e9lectronique \u00e0 balayage. Ces apprentissages se divisent en deux parties. La premi\u00e8re partie, th\u00e9orique, portera sur les protocoles de pr\u00e9paration (point critique et cong\u00e9lation haute pression) et sur le fonctionnement d\u2019un microscope \u00e9lectronique \u00e0 balayage. La seconde sera [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6776,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[205],"tags":[],"class_list":["post-6775","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-formation"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Formation Microscopie \u00c9lectronique \u00e0 Balayage - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/electron-microscopy-with-scanning\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Formation Microscopie \u00c9lectronique \u00e0 Balayage - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Cette formation vous permettra d\u2019acqu\u00e9rir le comp\u00e9tences pour la pr\u00e9paration des \u00e9chantillons et pour l\u2019observation en microscopie \u00e9lectronique \u00e0 balayage. Ces apprentissages se divisent en deux parties. La premi\u00e8re partie, th\u00e9orique, portera sur les protocoles de pr\u00e9paration (point critique et cong\u00e9lation haute pression) et sur le fonctionnement d\u2019un microscope \u00e9lectronique \u00e0 balayage. La seconde sera [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/electron-microscopy-with-scanning\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2021-07-02T16:07:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-11-20T14:55:44+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/montageformatipon-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1028\" \/>\n\t<meta property=\"og:image:height\" content=\"445\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"Formation Microscopie \u00c9lectronique \u00e0 Balayage\",\"datePublished\":\"2021-07-02T16:07:01+00:00\",\"dateModified\":\"2025-11-20T14:55:44+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/\"},\"wordCount\":214,\"publisher\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1.jpg\",\"articleSection\":[\"Formation\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"event\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/\",\"name\":\"Formation Microscopie \u00c9lectronique \u00e0 Balayage\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/#primaryimage\"},\"image\":[\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1-150x65.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1-300x130.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1-1024x443.jpg\",\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1.jpg\"],\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2021\\\/07\\\/montageformatipon-1.jpg\",\"datePublished\":\"2021-07-02T16:07:01+02:00\",\"dateModified\":\"2025-11-20T14:55:44+02:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/trigenotoul.com\\\/formation-microscopie-electronique-a-balayage\\\/\"]}],\"@context\":\"https:\\\/\\\/schema.org\",\"startDate\":\"2021-10-19\",\"endDate\":\"2021-10-20\",\"eventStatus\":\"EventScheduled\",\"eventAttendanceMode\":\"OfflineEventAttendanceMode\",\"location\":{\"@type\":\"place\",\"name\":\"CMEAB &amp; MeTi (UT3 - Rangeuil, Toulouse)\",\"address\":\"CMEAB &amp; MeTi (UT3 - Rangeuil, Toulouse)\",\"geo\":{\"@type\":\"GeoCoordinates\",\"latitude\":\"43.56839\",\"longitude\":\"1.46323\"}},\"description\":\"[caption id=\\\"attachment_6776\\\" align=\\\"alignleft\\\" width=\\\"561\\\"] Visualisation 3D de g\u00e9losomes dans un hydrogel par cryo fixation haute pression[\\\/caption]\\nCette formation vous permettra d\u2019acqu\u00e9rir le comp\u00e9tences pour la pr\u00e9paration des \u00e9chantillons et pour l\u2019observation en microscopie \u00e9lectronique \u00e0 balayage. Ces apprentissages se divisent en deux parties. La premi\u00e8re partie, th\u00e9orique, portera sur les protocoles de pr\u00e9paration (point critique et cong\u00e9lation haute pression) et sur le fonctionnement d\u2019un microscope \u00e9lectronique \u00e0 balayage. La seconde sera quant \u00e0 elle consacr\u00e9e \u00e0 des travaux pratiques de pr\u00e9paration et d'observation d'\u00e9chantillons.\\nCours (3h) :\\n \\tPr\u00e9paration des \u00e9chantillons\\n \\tPrincipe du MEB et de l\u2019acquisition des images\\n \\tExemples d\u2019application au MEB\\nTravaux Pratiques (13h) :\\n \\tPr\u00e9paration d\u2019\u00e9chantillons biologiques par la m\u00e9thode du point critique\\n \\tPr\u00e9paration d\u2019\u00e9chantillons biologique par cryo fixation haute pression\\n \\tObservation au MEB des \u00e9chantillons\\n \\tObservation d\u2019\u00e9chantillons au cryo-MEB\\nInformations et inscriptions \\n \\tDur\u00e9e : 2 jours\\n \\tPublic : Technicien, ing\u00e9nieur, chercheur de laboratoire de recherche public ou priv\u00e9.\\n \\tPr\u00e9requis : Connaissance en biologie ou chimie.\\n \\tPlateau : CMEAB (UT3 - Rangueil - Toulouse), pour les cours et l\u2019observation au MEB. 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Ces apprentissages se divisent en deux parties. La premi\u00e8re partie, th\u00e9orique, portera sur les protocoles de pr\u00e9paration (point critique et cong\u00e9lation haute pression) et sur le fonctionnement d\u2019un microscope \u00e9lectronique \u00e0 balayage. La seconde sera quant \u00e0 elle consacr\u00e9e \u00e0 des travaux pratiques de pr\u00e9paration et d'observation d'\u00e9chantillons.\nCours (3h) :\n \tPr\u00e9paration des \u00e9chantillons\n \tPrincipe du MEB et de l\u2019acquisition des images\n \tExemples d\u2019application au MEB\nTravaux Pratiques (13h) :\n \tPr\u00e9paration d\u2019\u00e9chantillons biologiques par la m\u00e9thode du point critique\n \tPr\u00e9paration d\u2019\u00e9chantillons biologique par cryo fixation haute pression\n \tObservation au MEB des \u00e9chantillons\n \tObservation d\u2019\u00e9chantillons au cryo-MEB\nInformations et inscriptions \n \tDur\u00e9e : 2 jours\n \tPublic : Technicien, ing\u00e9nieur, chercheur de laboratoire de recherche public ou priv\u00e9.\n \tPr\u00e9requis : Connaissance en biologie ou chimie.\n \tPlateau : CMEAB (UT3 - Rangueil - Toulouse), pour les cours et l\u2019observation au MEB. MeTi (UT3 - Rangueil - Toulouse) pour la cryofixation.\n \tRenseignement et inscription aupr\u00e8s de Bruno Payre","offers":{"@type":"Offer"}},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/trigenotoul.com\/formation-microscopie-electronique-a-balayage\/#primaryimage","url":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/montageformatipon-1.jpg","contentUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2021\/07\/montageformatipon-1.jpg","width":1028,"height":445,"caption":"Visualisation 3D de g\u00e9losomes dans un hydrogel par cryo fixation haute pression"},{"@type":"BreadcrumbList","@id":"https:\/\/trigenotoul.com\/formation-microscopie-electronique-a-balayage\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/trigenotoul.com\/"},{"@type":"ListItem","position":2,"name":"Formation Microscopie \u00c9lectronique \u00e0 Balayage"}]},{"@type":"WebSite","@id":"https:\/\/trigenotoul.com\/#website","url":"https:\/\/trigenotoul.com\/","name":"TRI-Genotoul","description":"Imaging, cytometry &amp; data analysis for the life sciences","publisher":{"@id":"https:\/\/trigenotoul.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/trigenotoul.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/trigenotoul.com\/#organization","name":"TRI-Genotoul","url":"https:\/\/trigenotoul.com\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/trigenotoul.com\/#\/schema\/logo\/image\/","url":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2017\/01\/ImagerieTRI_logo-Q3-1.png","contentUrl":"https:\/\/trigenotoul.com\/wp-content\/uploads\/2017\/01\/ImagerieTRI_logo-Q3-1.png","width":1000,"height":273,"caption":"TRI-Genotoul"},"image":{"@id":"https:\/\/trigenotoul.com\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/trigenotoul.com\/#\/schema\/person\/71018a7e00cf92ab28949e5339a08591","name":"gesteve","image":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/secure.gravatar.com\/avatar\/3ff90361fbad07b87db6e94afed78c806805bda9f3278f580caffd3dfd7e27e9?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/3ff90361fbad07b87db6e94afed78c806805bda9f3278f580caffd3dfd7e27e9?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/3ff90361fbad07b87db6e94afed78c806805bda9f3278f580caffd3dfd7e27e9?s=96&d=mm&r=g","caption":"gesteve"},"sameAs":["https:\/\/trigenotoul.com\/newsite"],"url":"https:\/\/trigenotoul.com\/en\/author\/gesteve\/"}]}},"_links":{"self":[{"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/posts\/6775","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/comments?post=6775"}],"version-history":[{"count":0,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/posts\/6775\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/media\/6776"}],"wp:attachment":[{"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/media?parent=6775"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/categories?post=6775"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/trigenotoul.com\/en\/wp-json\/wp\/v2\/tags?post=6775"}],"curies":[{"name":"well played","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}