{"id":6170,"date":"2019-11-26T14:22:22","date_gmt":"2019-11-26T13:22:22","guid":{"rendered":"https:\/\/trigenotoul.com\/?p=6170"},"modified":"2019-11-26T14:22:22","modified_gmt":"2019-11-26T13:22:22","slug":"j-morgado-brajones","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/j-morgado-brajones\/","title":{"rendered":"J. MORGADO BRAJONES: PhD thesis defence on OPTO-MECA-SPIM"},"content":{"rendered":"<h5 style=\"text-align: justify;\"><img decoding=\"async\" class=\"alignleft wp-image-6171\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2019\/11\/spimmeca.jpg\" alt=\"\" width=\"244\" height=\"150\" \/>Javier MORGADO BRAJONES will defend his thesis entitled \u00abOPTO-MECA-SPIM: Dynamic and interactive imaging of mechanobiological phenomena in tissues\u00bb on 9 December next in the seminar room of the Pierre Potier centre.<\/h5>\n<p>&nbsp;<\/p>\n<hr \/>\n<p style=\"text-align: justify;\">In recent years, tissue mimics (TMs) such as microtissues, spheroids, and organoid cultures have become increasingly important in life-science research, as they provide a physiologically relevant environment for cell growth, tissue morphogenesis, and stem cell differentiation. Selective Plane Illumination Microscopy (SPIM) is one of the most prominent microscopy modalities for three-dimensional tissue imaging, and an indispensable tool to understand cell biology in TMs. However, while SPIM is regarded as a very powerful tool for TM imaging, optical microscopy suffers from certain limitations when imaging 3D samples. Indeed, scattering, absorption and optical aberrations limit the depth at which useful imaging can be done, typically no more than 100 \u00b5m. Adaptive Optics (AO) is a technique capable of improving image quality at depth by correcting the optical aberrations introduced by the sample, which is seeing increasing use in fluorescence microscopes.<\/p>\n<p style=\"text-align: justify;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignleft  wp-image-6172\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2019\/11\/waospim-e1574774513465.jpg\" alt=\"\" width=\"667\" height=\"492\" \/>For this thesis, I have incorporated a wavefront sensor AO scheme to SPIM, able to correct aberrations in optically thick TMs such as multi-cellular tumour spheroids (MCTS). Due to the low amount of light produced by non-linear guide stars such as the one used in our system, a custom high-sensitivity Shack-Hartmann wavefront sensor (SHWFS) was developed for our needs. In this work, I characterise the performance of this SHWFS and the ability of our system to correct aberration in various conditions, including inside TMs.<\/p>\n<p style=\"text-align: justify;\">I show unprecedented image quality improvements for in-depth imaging of MCTS, regarding high-frequency detail and resolution. This allowed us to identify biologically relevant features at depths inaccessible to conventional SPIM.<\/p>\n<p style=\"text-align: justify;\">Up-converting nanoparticles (UCNPs) are rare-earth-based particles that are able to undergo photon up-conversion when illuminated, emitting light of a shorter wavelength than that of the illumination. Guide stars made from UCNPs are especially attractive due to the possibility of them being excited in the near-infrared while emitting visible light, reducing photodamage produced by the illumination light. The viability of using UCNPs as guide stars in biological samples is explored in this thesis.<\/p>","protected":false},"excerpt":{"rendered":"<p>Javier MORGADO BRAJONES soutiendra le 9 d\u00e9cembre prochain dans la salle de s\u00e9minaire du centre Pierre Potier sa th\u00e8se intitul\u00e9e \u00ab\u00a0OPTO-MECA-SPIM : Imagerie dynamique et interactive de ph\u00e9nom\u00e8nes m\u00e9canobiologiques dans les tissus\u00a0\u00bb. &nbsp; In recent years, tissue mimics (TMs) such as microtissues, spheroids, and organoid cultures have become increasingly important in life-science research, as they [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6171,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3],"tags":[],"class_list":["post-6170","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-evenements"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>J. 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MORGADO BRAJONES : Soutenance de th\u00e8se sur l&#039;OPTO-MECA-SPIM - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Javier MORGADO BRAJONES soutiendra le 9 d\u00e9cembre prochain dans la salle de s\u00e9minaire du centre Pierre Potier sa th\u00e8se intitul\u00e9e \u00ab\u00a0OPTO-MECA-SPIM : Imagerie dynamique et interactive de ph\u00e9nom\u00e8nes m\u00e9canobiologiques dans les tissus\u00a0\u00bb. &nbsp; In recent years, tissue mimics (TMs) such as microtissues, spheroids, and organoid cultures have become increasingly important in life-science research, as they [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/j-morgado-brajones\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2019-11-26T13:22:22+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2019\/11\/spimmeca.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"400\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/j-morgado-brajones\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/j-morgado-brajones\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"J. 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Selective Plane Illumination Microscopy (SPIM) is one of the most prominent microscopy techniques for three-dimensional tissue imaging, and an indispensable tool for understanding cell biology in TMs. However, whilst SPIM is regarded as a very powerful tool for TM imaging, optical microscopy suffers from certain limitations when imaging 3D samples. Indeed, scattering, absorption and optical aberrations limit the depth at which useful imaging can be achieved, typically to no more than 100 \u03bcm. Adaptive Optics (AO) is a technique capable of improving image quality at depth by correcting the optical aberrations introduced by the sample, and is seeing increasing use in fluorescence microscopes.\nFor this thesis, I have incorporated a wavefront sensor-based AO scheme into SPIM, capable of correcting aberrations in optically thick TMs such as multi-cellular tumour spheroids (MCTS). Due to the low light output from non-linear guide stars, such as the one used in our system, a custom high-sensitivity Shack-Hartmann wavefront sensor (SHWFS) was developed to meet our requirements. In this work, I characterise the performance of this SHWFS and the ability of our system to correct aberrations under various conditions, including inside tissue samples.\nI demonstrate unprecedented improvements in image quality for in-depth imaging of MCTS, in terms of high-frequency detail and resolution. This enabled us to identify biologically relevant features at depths inaccessible to conventional SPIM.\nUp-converting nanoparticles (UCNPs) are rare-earth-based particles capable of undergoing photon up-conversion when illuminated, emitting light of a shorter wavelength than that of the illumination. 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