{"id":4880,"date":"2017-09-13T12:06:02","date_gmt":"2017-09-13T10:06:02","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=4880"},"modified":"2017-09-13T12:06:02","modified_gmt":"2017-09-13T10:06:02","slug":"a-high-resolution-airyscan-module-at-the-li2mc","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/un-module-haute-resolution-airyscan-a-li2mc\/","title":{"rendered":"An Airyscan high-resolution module at the I2MC"},"content":{"rendered":"
After the Elyra PS.1 super-resolution<\/a>, the I2MC Imaging facility has acquired a new high-resolution module Airyscan<\/strong> implemented on an LSM 780 (Zeiss) confocal microscope.<\/h5>\n

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\"\"In addition to its two standard PMT detectors, this microscope is equipped with a high-sensitivity GaAsP-type detector. Thanks to its quantum efficiency, which is twice as effective as that of a standard PMT, this microscope is capable of acquiring images from low light intensities. Building on this sensitivity, the installation of the Airyscan enables the acquisition of high-resolution images (140 nm laterally (xy) compared to 200 nm in confocal mode).<\/p>\n

The Airyscan detector is particularly useful when the sample, due to weak and\/or labile labelling, cannot be imaged in super-resolution (risk of photobleaching). Finally, the ability to switch from confocal to Airyscan mode on the same sample (fixed or living) makes it easier to use.<\/p>\n