{"id":4842,"date":"2017-09-17T09:23:54","date_gmt":"2017-09-17T07:23:54","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=4842"},"modified":"2017-09-17T09:23:54","modified_gmt":"2017-09-17T07:23:54","slug":"3d-architectures-of-cellular-colonizations","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/architectures-3d-colonisations-cellulaires\/","title":{"rendered":"3D architectures & cellular colonizations\u2026"},"content":{"rendered":"
3D architectures are essential tools that, by recreating environments of biomimetic cellular cultures, allow the study of cell growth, proliferation, and differentiation. Researchers from the LAAS (CNRS), in partnership with the TRI-Genotoul platforms of the CPTP and Restore, have managed, using laser engraving performed using multiphoton microscopes, to fabricate self-supporting 3D scaffolds. These scaffolds were then seeded with neural cells and characterized using advanced approaches of 3D fluorescence imaging. The results published in the journal Small<\/strong> The results of Advanced Science news are particularly encouraging.<\/h5>\n

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Matrix created by the researchers<\/figcaption><\/figure>\n

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The technique employed here allows for a much finer manufacturing process (\u2248 200 nm) than conventional 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser drying, or the \u00abpolyjet\u00bb technology.<\/p>\n

The samples thus created were then studied using scanning electron microscopy (SEM), which revealed the morphology of the cells and the cellular connections around the 3D architecture. The positioning of the nucleus, inaccessible by conventional SEM imaging, is revealed by light sheet fluorescence microscopy and multiphoton confocal imaging. These different techniques have highlighted long neural extensions and optimal cellular colonization around and within the 3D scaffold. These new architectures and characterization techniques open up interesting perspectives for both neural cell culture and observation.<\/p>\n

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Consult the journal's website<\/strong><\/a><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

Les architectures 3D sont des outils essentiels qui, en recr\u00e9ant\u00a0des environnements de cultures cellulaires biomim\u00e9tiques, permettant l’\u00e9tude de la croissance cellulaire, de la prolif\u00e9ration et de la diff\u00e9renciation. Des chercheurs du LAAS (CNRS), en partenariat avec les plateformes de TRI-Genotoul du CPTP et de l’Restore, sont parvenus, gr\u00e2ce au gravage laser r\u00e9alis\u00e9s \u00e0 l’aide de […]<\/p>\n","protected":false},"author":1,"featured_media":4844,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4842","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"\nArchitectures 3D & colonisations cellulaires... - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/3d-architectures-of-cellular-colonizations\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Architectures 3D & colonisations cellulaires... - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Les architectures 3D sont des outils essentiels qui, en recr\u00e9ant\u00a0des environnements de cultures cellulaires biomim\u00e9tiques, permettant l’\u00e9tude de la croissance cellulaire, de la prolif\u00e9ration et de la diff\u00e9renciation. Des chercheurs du LAAS (CNRS), en partenariat avec les plateformes de TRI-Genotoul du CPTP et de l’Restore, sont parvenus, gr\u00e2ce au gravage laser r\u00e9alis\u00e9s \u00e0 l’aide de […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/3d-architectures-of-cellular-colonizations\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2017-09-17T07:23:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2017\/06\/matrice-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"664\" \/>\n\t<meta property=\"og:image:height\" content=\"635\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"Architectures 3D & colonisations cellulaires…\",\"datePublished\":\"2017-09-17T07:23:54+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\"},\"wordCount\":311,\"publisher\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2017\\\/06\\\/matrice-1.jpg\",\"articleSection\":[\"Actualit\u00e9s\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\",\"name\":\"Architectures 3D & colonisations cellulaires... - 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