{"id":4842,"date":"2017-09-17T09:23:54","date_gmt":"2017-09-17T07:23:54","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=4842"},"modified":"2017-09-17T09:23:54","modified_gmt":"2017-09-17T07:23:54","slug":"architectures-3d-colonisations-cellulaires","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/architectures-3d-colonisations-cellulaires\/","title":{"rendered":"Architectures 3D & colonisations cellulaires…"},"content":{"rendered":"
Les architectures 3D sont des outils essentiels qui, en recr\u00e9ant\u00a0des environnements de cultures cellulaires biomim\u00e9tiques, permettant l’\u00e9tude de la croissance cellulaire, de la prolif\u00e9ration et de la diff\u00e9renciation. Des chercheurs du LAAS (CNRS), en partenariat avec les plateformes de TRI-Genotoul du CPTP et de l’Restore, sont parvenus, gr\u00e2ce au gravage laser r\u00e9alis\u00e9s \u00e0 l’aide de microscopes multiphotons, \u00e0 fabriquer des \u00e9chafaudages 3D autoportants. Ces supports ont \u00e9t\u00e9 ensuite\u00a0 ensemenc\u00e9s des cellules neurales puis caract\u00e9ris\u00e9s \u00e0 l’aide d’approches avanc\u00e9es d’imagerie par fluorescence 3D. Les r\u00e9sultats publi\u00e9s dans la revue Small<\/strong> de Advanced Science news se r\u00e9v\u00e8lent particuli\u00e8rement encourageants.<\/h5>\n

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\"Matrice
Matrice cr\u00e9\u00e9e par les chercheurs<\/figcaption><\/figure>\n

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La technique ici employ\u00e9e, autorise un processus de fabrication beaucoup plus fin (\u2248 200 nm) que les technologies habituelles d’impression 3D classiques bas\u00e9es sur la mod\u00e9lisation de d\u00e9p\u00f4ts fusionn\u00e9s, l’impression jet d’encre, le s\u00e9chage s\u00e9lectif au laser ou la technologie \u00ab\u00a0polyjet\u00a0\u00bb.<\/p>\n

Les \u00e9chantillons ainsi cr\u00e9\u00e9s ont ensuite \u00e9t\u00e9 \u00e9tudi\u00e9s par de la microscopie \u00e9lectronique \u00e0 balayage (SEM) pr\u00e9cisant ainsi la morphologie des cellules et les connexions cellulaires autour de l’architecture 3D. Le positionnement du noyau, inaccessible par l’imagerie SEM conventionnelle, est quant \u00e0 lui d\u00e9voil\u00e9 par microscopie de fluorescence en feuille de lumi\u00e8re et imagerie confocale multiphotonique. Ces diff\u00e9rentes techniques ont mis en \u00e9vidence de longues extensions neuritiques et une colonisation cellulaire optimale autour et \u00e0 l’int\u00e9rieur de l’\u00e9chafaudage 3D. Ces nouvelles architectures et techniques de caract\u00e9risation ouvrent des perspectives int\u00e9ressantes \u00e0 la fois pour la culture et l’observation de cellules neurales.<\/p>\n

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Consulter sur le site de la revue<\/strong><\/a><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

Les architectures 3D sont des outils essentiels qui, en recr\u00e9ant\u00a0des environnements de cultures cellulaires biomim\u00e9tiques, permettant l’\u00e9tude de la croissance cellulaire, de la prolif\u00e9ration et de la diff\u00e9renciation. Des chercheurs du LAAS (CNRS), en partenariat avec les plateformes de TRI-Genotoul du CPTP et de l’Restore, sont parvenus, gr\u00e2ce au gravage laser r\u00e9alis\u00e9s \u00e0 l’aide de […]<\/p>\n","protected":false},"author":1,"featured_media":4844,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4842","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"\nArchitectures 3D & colonisations cellulaires... - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/architectures-3d-colonisations-cellulaires\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Architectures 3D & colonisations cellulaires... - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Les architectures 3D sont des outils essentiels qui, en recr\u00e9ant\u00a0des environnements de cultures cellulaires biomim\u00e9tiques, permettant l’\u00e9tude de la croissance cellulaire, de la prolif\u00e9ration et de la diff\u00e9renciation. Des chercheurs du LAAS (CNRS), en partenariat avec les plateformes de TRI-Genotoul du CPTP et de l’Restore, sont parvenus, gr\u00e2ce au gravage laser r\u00e9alis\u00e9s \u00e0 l’aide de […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/architectures-3d-colonisations-cellulaires\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2017-09-17T07:23:54+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2017\/06\/matrice-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"664\" \/>\n\t<meta property=\"og:image:height\" content=\"635\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"Architectures 3D & colonisations cellulaires…\",\"datePublished\":\"2017-09-17T07:23:54+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\"},\"wordCount\":311,\"publisher\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2017\\\/06\\\/matrice-1.jpg\",\"articleSection\":[\"Actualit\u00e9s\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/architectures-3d-colonisations-cellulaires\\\/\",\"name\":\"Architectures 3D & colonisations cellulaires... - 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