{"id":4596,"date":"2017-01-31T15:46:57","date_gmt":"2017-01-31T14:46:57","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=4596"},"modified":"2017-01-31T15:46:57","modified_gmt":"2017-01-31T14:46:57","slug":"imagestreamx-cytometer-and-microscope","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/imagestreamx-cytometer-and-microscope\/","title":{"rendered":"ImageStreamX : Imaging Flow Cytometer"},"content":{"rendered":"<h5><img fetchpriority=\"high\" decoding=\"async\" class=\"alignleft wp-image-3082\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/09\/isx_2-1024x753.jpg\" alt=\"isx_2\" width=\"353\" height=\"324\" \/>The ImageStreamX imaging flow cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This system delivers multiple images of every cell in flow, including brightfield, darkfield (SSC) and fluorescent markers. The camera operates with a pixel size of 0.1\/0.25\/1 \u00b5m2 with 60X\/40X\/20X magnification, respectively, allowing visualisation of fluorescence location from the membrane, cytoplasm, subcellular organelles or nucleus at high resolution. This instrument opens the door to an extensive range of applications in immunology, oncology, microbiology, parasitology\u2026 These applications include the study of cell-cell interactions, internalisation, nuclear translocation, spot counting, apoptosis\u2026<\/h5>\n<hr \/>\n<p>&nbsp;<\/p>\n<h5>Technical characteristics:<\/h5>\n<ul>\n<li>Illumination:\n<ul>\n<li>Excitation: 405nm, 488nm and 642nm<\/li>\n<li>Side scatter : 785nm<\/li>\n<li>brightfield : multichannel<\/li>\n<\/ul>\n<\/li>\n<li>6-channel images are collected using a CCD camera operated in TDI (time delay integration) mode<\/li>\n<li>3 objectives: 20x, 40x and 60x,<\/li>\n<li>2 software: INSPIRE (acquisition) and IDEAS (analysis)<\/li>\n<\/ul>\n<hr \/>\n<p>&nbsp;<\/p>\n<h5><img decoding=\"async\" class=\"wp-image-3081 alignright\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/09\/ISXpicture-1024x433.jpg\" alt=\"ISXpicture\" width=\"561\" height=\"296\" \/>Applications:<\/h5>\n<ul>\n<li>Morphological analysis<\/li>\n<li>Internalisation<\/li>\n<li>Nuclear translocation<\/li>\n<li>Colocalisation<\/li>\n<li>Cellular interactions<\/li>\n<li>Cell cycle<\/li>\n<li>Apoptosis<\/li>\n<li>Spotting<\/li>\n<li>Autophagy<\/li>\n<li>Emerging applications (FISH, FRET\u2026)<\/li>\n<\/ul>\n<hr \/>\n<p>&nbsp;<\/p>\n<p>Contact: <strong><a href=\"mailto:valerie.duplan@inserm.fr\">Val\u00e9rie Duplan-Eche<\/a><\/strong> \/ + 33 531 547 911<\/p>\n<p>Location: <strong><a title=\"OUR PLATFORMS\" href=\"https:\/\/trigenotoul.com\/en\/tri-genotoul-2\/our-platforms\/\">Infinity<\/a><\/strong><\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>The ImageStreamX imaging flow cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This system delivers multiple images of every cell in flow, including brightfield, darkfield (SSC) and fluorescent markers. The camera operates with a pixel size of 0.1\/0.25\/1 \u03bcm2 with 60X\/40X\/20X magnification, respectively, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":3082,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4596","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>ImageStreamX : Imaging Flow Cytometer - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/imagestreamx-cytometer-and-microscope\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ImageStreamX : Imaging Flow Cytometer - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"The ImageStreamX imaging flow cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. 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