{"id":3779,"date":"2016-05-11T16:05:43","date_gmt":"2016-05-11T14:05:43","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=3779"},"modified":"2016-05-11T16:05:43","modified_gmt":"2016-05-11T14:05:43","slug":"tri-invest-superresolution-systems","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/tri-invest-superresolution-systems\/","title":{"rendered":"TRI invests in super-resolution systems"},"content":{"rendered":"<h5 style=\"text-align: justify;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignright size-medium wp-image-3317\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/11\/superres-450x218.jpg\" alt=\"Actin filaments \u00a9T. Mangeat &amp; A. Negash\" width=\"450\" height=\"218\" align=\"right\" \/>In order to overcome the resolution limits imposed by <a href=\"#loisoptiques\">laws of optics<\/a> and thus to achieve higher resolutions, various fluorescence microscopy technologies have recently been developed. Grouped together under the terms \u00absuper-resolution\u00bb microscopy or \u00abnanoscopy\u00bb, they make it possible to localise, in vivo, right inside cells, compounds on the single-molecule scale (lateral resolutions of the order of 10 to 90 nm). These technologies, which rely either on principles similar to pointillism (PALM\/STORM) or on structured illumination (SIM) or laser depletion (STED), earned their authors, E. Betzig, S. Hell and W. Moerner, the Nobel Prize in Chemistry in 2014.<\/h5>\n<p>&nbsp;<\/p>\n<blockquote>\n<h5 style=\"text-align: justify;\">Conducting competitive research across many fields of biology now requires access to super-resolution microscopy, which is why TRI is developing a super-resolution microscopy hub that benefits from these various approaches and is open to the entire scientific community.<\/h5>\n<\/blockquote>\n<p>&nbsp;<\/p>\n<h3 style=\"text-align: justify;\">Industrial Equipment<\/h3>\n<hr \/>\n<ul style=\"list-style-type: square;\">\n<li style=\"text-align: justify;\">dSTORM and STED type equipment at the CPTP. The former develops single-colour dSTORM type approaches in TIRF microscopy, using a 400 mW 640 nm laser. As for the latter, the STED, its originality lies in the fact that it is the only super-resolution microscope enabling work on 3D tissue sections, thereby providing access to human physiopathology with very high resolution (30 nm).<\/li>\n<li style=\"text-align: justify;\">Similar equipment, the N-Storm, is operational within the platforms united under the common LICT (CBD \/ CBI) banner. It uses the same technologies as the previous ones but allows for observations in a wider variety of wavelengths.<\/li>\n<li style=\"text-align: justify;\">A Zeiss ELYRA PS1 type equipment has been installed at the I2MC site. This super-resolution microscope uses a concept of nano-interaction analysis at the level of contacts between different cell types or between cells and the extracellular matrix. Thanks to this equipment, it is possible to analyse the same sample using the three technologies mentioned previously, SIM and STORM or PALM, in order to obtain an extremely detailed result, allowing single molecules to be localised with precision.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3 style=\"text-align: justify;\">R&amp;D technology<\/h3>\n<hr \/>\n<p style=\"text-align: justify;\">Two microscopes currently under development, based on a technique coupling 3D localisation and structured illumination microscopy (SIM), are operational at the LITC site. Integrating the latest innovations in structured illumination imaging from internationally renowned microscopy laboratories (IPHT \u2013 Germany, Institut Fresnel \u2013 France, ISMO \u2013 France, Janelia Lab \u2013 USA), they overcome the experimental constraints encountered in conventional super-resolution equipment. Technological support from the French companies Oxxius (laser technology) and AbbeLight (supercritical 3D localisation) will be effective throughout 2016.<\/p>\n<figure id=\"attachment_3334\" aria-describedby=\"caption-attachment-3334\" style=\"width: 800px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"wp-image-3334 size-large\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2016\/02\/super-resolution-1024x442.jpg\" alt=\"super-resolution\" width=\"800\" height=\"345\" \/><figcaption id=\"caption-attachment-3334\" class=\"wp-caption-text\"><strong>On the left:<\/strong> The R&amp;D Laser Chain (Photo D. Villa). <strong>On the right<\/strong> a\/ Principle of structured illumination with 9 high-contrast grids at the optical resolution limit for 2D SIM imaging. b\/ Resolution extension using grid-assisted deconvolution methods. Bottom: Example of conventional and super-resolved imaging at 80nm focal adhesion resolution (scale bar 640nm).<\/figcaption><\/figure>\n<p style=\"text-align: justify;\"><strong> These items of equipment may be coupled with existing techniques such as optomanipulation:<\/strong><\/p>\n<ul>\n<li style=\"text-align: justify;\">Conventional 2D TIRF-SIM and 3D SIM at a temporal frequency of 20 frames per second. This technique will thus enable super-resolution imaging of rapid phenomena such as mitosis and endocytosis,<\/li>\n<li style=\"text-align: justify;\">Fast 2D non-linear SIM enabling a resolution of 50nm using photo-activatable GFP molecules (acquisition rate: 5 frames \/ s). It will be dedicated to cytoskeleton and chromatin imaging (available in spring 2016),<\/li>\n<li style=\"text-align: justify;\">Single-objective multicolour isotropic 3D SIM imaging to democratise super-resolved isotropic imaging using a conventional microscope stand (available at the end of 2016),<\/li>\n<li style=\"text-align: justify;\">2D SIM TIRF imaging combined with 3D localisation via supercritical angle fluorescence, in collaboration with AbbeLight (implemented in July 2016). It will enable a resolution close to 20nm in Z to be achieved,<\/li>\n<li style=\"text-align: justify;\">3D SIM reconstruction via a new SIM deconvolving method adapted for live tissues (BLIND SIM), not available through commercial algorithms.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><a id=\"loisoptiques\"><\/a>* \u00ab\u00a0<strong>From microscopy to nanoscopy<\/strong>\u00ab, M. Moreau, P. Cochard, m\/s no. 12, vol. 30, December 2014<\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>Afin de s\u2019affranchir des limites de r\u00e9solution impos\u00e9es par les lois de l\u2019optique* et ainsi atteindre de plus hautes r\u00e9solutions, diff\u00e9rentes technologies de microscopie de fluorescence ont \u00e9t\u00e9 r\u00e9cemment d\u00e9velopp\u00e9es. Regroup\u00e9es sous les termes de microscopie de \u00ab super-r\u00e9solution \u00bb ou \u00ab nanoscopie \u00bb, elles permettent de localiser, in vivo, au sein m\u00eame des cellules, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":3187,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[5],"tags":[],"class_list":["post-3779","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-en"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>TRI invest superresolution systems - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/tri-invest-superresolution-systems\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"TRI invest superresolution systems - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Afin de s\u2019affranchir des limites de r\u00e9solution impos\u00e9es par les lois de l\u2019optique* et ainsi atteindre de plus hautes r\u00e9solutions, diff\u00e9rentes technologies de microscopie de fluorescence ont \u00e9t\u00e9 r\u00e9cemment d\u00e9velopp\u00e9es. Regroup\u00e9es sous les termes de microscopie de \u00ab super-r\u00e9solution \u00bb ou \u00ab nanoscopie \u00bb, elles permettent de localiser, in vivo, au sein m\u00eame des cellules, [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/trigenotoul.com\/en\/tri-invest-superresolution-systems\/\" \/>\n<meta property=\"og:site_name\" content=\"TRI-Genotoul\" \/>\n<meta property=\"article:published_time\" content=\"2016-05-11T14:05:43+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/11\/Leica-TCS-SP8-STED-3X-Brochure_EN-7-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"993\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"gesteve\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"gesteve\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/\"},\"author\":{\"name\":\"gesteve\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#\\\/schema\\\/person\\\/71018a7e00cf92ab28949e5339a08591\"},\"headline\":\"TRI invest superresolution systems\",\"datePublished\":\"2016-05-11T14:05:43+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/\"},\"wordCount\":786,\"publisher\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2015\\\/11\\\/Leica-TCS-SP8-STED-3X-Brochure_EN-7-1.jpg\",\"articleSection\":[\"News\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/\",\"name\":\"TRI invest superresolution systems - TRI-Genotoul\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2015\\\/11\\\/Leica-TCS-SP8-STED-3X-Brochure_EN-7-1.jpg\",\"datePublished\":\"2016-05-11T14:05:43+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#primaryimage\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2015\\\/11\\\/Leica-TCS-SP8-STED-3X-Brochure_EN-7-1.jpg\",\"contentUrl\":\"https:\\\/\\\/trigenotoul.com\\\/wp-content\\\/uploads\\\/2015\\\/11\\\/Leica-TCS-SP8-STED-3X-Brochure_EN-7-1.jpg\",\"width\":1000,\"height\":993,\"caption\":\"Immunofluorescence staining in HeLa cells: red: NUP 153, green: Clathrin-TMR. gSTED with 0% 3D STED - \u00a9Leica\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/tri-invest-superresolution-systems\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/trigenotoul.com\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"TRI invest superresolution systems\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/trigenotoul.com\\\/#website\",\"url\":\"https:\\\/\\\/trigenotoul.com\\\/\",\"name\":\"TRI-Genotoul\",\"description\":\"Imagerie, cytom\u00e9trie &amp; 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