{"id":3080,"date":"2015-09-18T12:45:34","date_gmt":"2015-09-18T10:45:34","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=3080"},"modified":"2015-09-18T12:45:34","modified_gmt":"2015-09-18T10:45:34","slug":"imagestreamx-cytometer-and-microscope-2","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/limagestreamx-cytometre-et-microscope\/","title":{"rendered":"The ImageStreamX: Imaging Cytometer"},"content":{"rendered":"<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td rowspan=\"3\">\n<h3 style=\"text-align: justify;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignleft wp-image-3082\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/09\/isx_2.jpg\" alt=\"Image stream X\" width=\"392\" height=\"227\" \/><\/h3>\n<\/td>\n<td>\n<h3 style=\"text-align: justify;\">Characterising and observing cell populations<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td><a href=\"mailto:cytometrie@genotoul.fr?subject=TRI : ImagestreamX\"><img decoding=\"async\" class=\"alignright wp-image-249 size-full\" title=\"Contact &amp; booking\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2014\/06\/r\u00e9server.png\" alt=\"Click here to book this resource\" width=\"159\" height=\"69\" \/><\/a><\/p>\n<div align=\"right\">\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h5 style=\"text-align: left;\"><img decoding=\"async\" class=\"alignnone wp-image-494\" title=\"valign=middle\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2014\/06\/telephone.png\" alt=\"Telephone:\" width=\"18\" height=\"18\" align=\"texttop\" \/><span class=\"phone cont-info\"> 0<\/span>5 31 54 79 11<\/h5>\n<h4 style=\"text-align: justify;\"><\/h4>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h5>The ImageStreamX is an interdisciplinary instrument that bridges flow cytometry and microscopy. It combines the advantages of both technologies, thereby bringing the power of statistical flow cytometry data to a large number of cellular morphological parameters. This system generates images of cells in flow using magnifications ranging from 20x to 60x. It caters to a wide range of applications (spot counting, nuclear translocation, internalisation, etc.) across various fields: immunology, oncology, parasitology, microbiology, etc.<\/h5>\n<hr \/>\n<h5>Technical specifications:<\/h5>\n<ul>\n<li>a Brightfield laser enabling the measurement of cell size and morphology,<\/li>\n<li>a 785 nm darkfield laser providing information on the cell's content and granularity (side scatter),<\/li>\n<li>3 lasers enabling the quantification and localisation of markers of interest in each detection channel: 405nm, 488nm and 642nm,<\/li>\n<li>Equipped with a CCD camera, this configuration makes it possible to collect simultaneously up to 6 images of each cell,<\/li>\n<li>3 objective lens sizes: 20x, 40x and 60x,<\/li>\n<li>2 associated software programmes: INSPIRE (acquisition) and IDEAS (statistical analysis).<\/li>\n<\/ul>\n<hr \/>\n<h5><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3081 alignright\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2015\/09\/ISXpicture-1024x433.jpg\" alt=\"ISXpicture\" width=\"561\" height=\"296\" \/>Applications:<\/h5>\n<ul>\n<li>morphological analysis,<\/li>\n<li>internalisation,<\/li>\n<li>cell signalling, nuclear translocation,<\/li>\n<li>colocalisation,<\/li>\n<li>cellular interactions,<\/li>\n<li>cell cycle,<\/li>\n<li>apoptosis,<\/li>\n<li>spot counting,<\/li>\n<li>autophagy and cell death,<\/li>\n<li>numerous emerging applications (FISH, FRET, etc.)<\/li>\n<\/ul>\n<hr \/>\n<h3>Access mode:<\/h3>\n<ul>\n<li>With assistance<\/li>\n<li>Collaborative R&amp;D project<\/li>\n<li>As service delivery<\/li>\n<\/ul>\n<hr \/>\n<h3>Our cytometers image resources<a href=\"mailto:cytometrie@genotoul.fr?subject=TRI : ImagestreamX\"><img decoding=\"async\" class=\"alignright wp-image-249 size-full\" title=\"Contact &amp; booking\" src=\"https:\/\/trigenotoul.com\/wp-content\/uploads\/2014\/06\/r\u00e9server.png\" alt=\"Click here to book this resource\" width=\"159\" height=\"69\" \/><\/a><\/h3>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td>\n<h5>Designation<\/h5>\n<\/td>\n<td>\n<h5><\/h5>\n<\/td>\n<td>\n<h5>Plateau<\/h5>\n<\/td>\n<\/tr>\n<tr>\n<td>Image streamX<\/td>\n<td><\/td>\n<td>Infinity- Purpan<\/td>\n<\/tr>\n<tr>\n<td>Image streamX<\/td>\n<td><\/td>\n<td>Restore \u2013 Oncop\u00f4le<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>Caract\u00e9riser et observer des populations de cellules &nbsp; &nbsp; 05 31 54 79 11 L\u2019ImageStreamX est un \u00e9quipement interdisciplinaire entre la cytom\u00e9trie et l\u2019imagerie. Il conjugue les avantages de ces 2 technologies et apporte ainsi la force des donn\u00e9es statistiques de la cytom\u00e9trie appliqu\u00e9e \u00e0 un grand nombre de param\u00e8tres morphologiques cellulaires. Ce syst\u00e8me g\u00e9n\u00e8re [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":3082,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3080","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-actualites-fr"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>L\u2019ImageStreamX : Cytom\u00e8tre en images - TRI-Genotoul<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/trigenotoul.com\/en\/imagestreamx-cytometer-and-microscope-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"L\u2019ImageStreamX : Cytom\u00e8tre en images - TRI-Genotoul\" \/>\n<meta property=\"og:description\" content=\"Caract\u00e9riser et observer des populations de cellules &nbsp; &nbsp; 05 31 54 79 11 L\u2019ImageStreamX est un \u00e9quipement interdisciplinaire entre la cytom\u00e9trie et l\u2019imagerie. 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