{"id":2771,"date":"2015-03-19T16:17:27","date_gmt":"2015-03-19T15:17:27","guid":{"rendered":"http:\/\/trigenotoul.com\/?p=2771"},"modified":"2015-03-19T16:17:27","modified_gmt":"2015-03-19T15:17:27","slug":"spim-macro","status":"publish","type":"post","link":"https:\/\/trigenotoul.com\/en\/macro-spim\/","title":{"rendered":"Macro-SPIM: A new imaging tool"},"content":{"rendered":"

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One of the challenges in biological imaging is to visualise biological structures and living organisms in three dimensions and in depth. After developing a first light-sheet microscope, the SPIM (Selective Plane Illumination Microscope), the Restore imaging platform is acquiring a variant of this device, the macro-SPIM<\/strong>.<\/p>\n

Just like its cousin, the SPIM, it allows for rapid, 3D and in-depth optical sectioning of a sample, whilst offering multi-view acquisition capabilities. Furthermore, unlike all the 3D imaging tools available on the market, image reconstructions obtained by light-sheet microscopy are free from any optical distortion. Conversely, unlike the SPIM, which is restricted to small samples (from a hundred micrometres to a millimetre), the macro-SPIM allows for acquisitions with large samples (on the scale of a centimetre), such as whole organs. Naturally, these organs must first undergo a \u00abtissue clearing\u00bb treatment.<\/p>\n

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It was a collaboration with Restore that brought the need to light. Confocal microscopy was used at the time, but the very long acquisition times constituted a serious obstacle... which has now been overcome thanks to this new resource.<\/p>\n