FRAP/PHOTOABLATION/OPTOGENETICS

TMangeat Station polyvalente

Versatility in handling living components

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Telephone : +33 5 61 55 75 93
Focused on the handling of living components, this highly versatile station serves in particular to quantify the exchanges between cellular compartments or to measure the protein diffusion rate according to different experimental conditions (“FRAP” techniques). For its part, optogenetics (or photoactivation) is a recent technique, originally used in neurology and whose areas of application are progressively expanding. It is used at the TRI platform in particular for activating and inactivating DNA transcription or protein production. Finally, photoablation makes it possible to use light as a knife to observe the fate or remodeling of DNA molecules, cell membranes and cytoskeletons.

 

Services :

  • Study of multi-point and multi-zone diffusion (FRAP)
  • Multi-point and multi-zone optogenetics
  • Nanodissection of cell cytoskeletons and apical cell membranes, etc.
  • DNA damage
  • Fluorescence acquisition and monitoring
  • Image analysis coupled with these techniques
  • Post-processing (deconvolution, realignment, monitoring, analysis of diffusion, etc.)

 

Access mode:

  • By assistance
  • Collaborative project
  • Service delivery

Break of filaments of actin by photoablation

 

Available resources

 

Name
Technical caracteristics
Location
FRAP/ photoblation/ optogénétique Mounted on inverted wide-field microscope coupled to a multi-point scanning laser system
FRAP laser at 473 nm
Photoablation in 0.4 ns at a frequency of 7 kHz		 LBCMCP – Campus UPS
FRAP/ Spinning Disk Mounted on an inverted spinning disk, FRAP laser at 375 nm Infinity – Purpan
FRAP/ photoablation Mounted on inverted wide-field microscope, micro-point laser illumination and ablation from 365 nm to 656 nm IBCG – UPS Campus

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Several publications made thanks to this resource:

  • Gemal-Cedrick Taty-Taty and all, Cell Cycle 13:3, 1-9; frebruary, 2014
  • G.gay, Courtheoux, T.,Reyes.C,Tournier.S and Gachet.Y. (2012). J Cell Biol 196 (6):757
  • Courtheoux, T., Gay, G. Gachet, Y., and Tournier, S. (2009). J Cell Biol 187, 399-412

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