CRYO-METHODS

Cryo methodes

Sample preparation techniques for electron microscopy

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Telephone : +33 5 62 88 90 35 / +33 5 61 33 59 12
This page brings together all of the resources that use sample preparation techniques at low temperature for a better morphological preservation. The samples thus prepared can be observed by transmission (TEM) or scanning (SEM) cryomicroscopy.

 

Cryosection de cellules tumorales fixées chimiquement (technique Tokuyasu) Observation à 80KV

Cryo-section of chemically fixed tumor cells (Tokuyasu technique) – Observation at 80 KV

Services:

  • Ultra-rapid freezing by immersion in liquid ethane, or by high pressure freezing of whole samples (bacteria, viruses, particles, liposomes, etc.) in vitreous ice up to 200 µm in thickness
  • Cryo-sections (CEMOVIS and Tokuyasu techniques)
  • Automated cryo-substitution and resin embedding at very low temperatures
  • Cryo-fracture and sublimation
  • Transmission and scanning cryomicroscopy

 

Access mode:

  • With assistance
  • As service delivery

 

Available resources in sample preparation for electron microscopy

 

Nom
Technical characteristics
Location
Cryo-ultramicrotome
Leica UC7/FC7
Removable cryo chamber, cryosphere (to avoid contamination by frost), antistatic system & micromanipulator METi – UPS Campus
Freeze substitution system
AFS-2/FSP
Fully automated processes, associated with an automated device to manage reagents METi – UPS Campus
Cryofixateur high pressure cryo-fixation system
Leica EMPACT
Freezing at -8000 to -12,000°/s under 2000 bars of pressure METi – UPS Campus
Cryoplunge
Leica EMGP
Automated device for ultra-rapid freezing in ethane, equipped with a stereomicroscope METi – UPS Campus
Cryopreparation module
PP3000T Quorum
Cryo transfer chamber for MEB FEG Quanta 250 FEI
for metallization, cryo-fracture and sublimation
CMEAB, Rangueil faculty of medecine

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Some publications made thanks to these resources:

  • Carron C, Balor S, Delavoie F, Plisson-Chastang C, Faubladier M, Gleizes PE, O’Donohue MF; J. Cell Science Octobre 2012, Post-mitotic dynamics of pre-nucleolar bodies is driven by pre-rRNA processing.
  • Rath P, Saurel O, Czaplicki G, Tropis M, Daffé M, Ghazi A, Demange P, Milon A.. Cord factor (trehalose 6,6′-dimycolate) forms fully stable and non-permeable lipid bilayers required for a functional outer membrane. Biochim Biophys Acta. Sep. 2013.
  • Artificial feeding of Varroa destructor through a chitosan membrane: a tool for studying the host-microparasite relationship. Tabart J1, Colin ME, Carayon JL, Tene N, Payre B, Vetillard A. Exp Appl Acarol, 2013,61(1), 107-18.

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